Commercial PCR kits are available, ready-to-use, and optimized for specific conditions to help take the guesswork out of research. Polymerase chain reaction (PCR), where DNA is amplified exponentially, is a standard yet powerful tool in the molecular biologist’s toolkit.
Many kits have been developed that utilize PCR or PCR-based techniques, including quantitative PCR (also known as qPCR or real-time PCR), reverse transcription PCR (RT-PCR), and quantitative reverse transcription PCR (RT-qPCR).
These convenient kits generally include all the basic ingredients needed for a successful PCR reaction, such as polymerase, deoxynucleotides (dNTPs), buffer, and magnesium chloride.
For PCR detection or assay kits, sequence-specific primer sets are generally included.
These kits can serve as useful tools in applications such as template amplification for sequencing and cloning, library generation, forensic identification, mutagenesis, expression profiling and amplification of numerous targets at one time (multiplexing).
PCR kits are optimized for specific conditions to help take the guesswork out of research.
In choosing an appropriate PCR kits it is important to consider the PCR platform in use, the desired fidelity, read length vs. turnaround time, and the downstream application.
As PCR can be a challenging complex process, it is important to work with a vendor with excellent, readily available technical support.
Recommended Reading:
-
Technological advances mixed with human creativity have produced many types of PCR, each with its benefits and challenges.
This article will review some widely used PCR methods amidst a pandemic, and give some examples of new PCR tools.
-
To get the best results from the polymerase chain reaction (PCR), scientists often rely on purification.
Performing that process as well as possible depends on information and the right tools.
A myriad of tools and technologies exist for exosome characterization.
Steps must be taken so data & intel is easily available to care teams.
The kit is used by the user to extract their target band or fragment post PCR or...
The reagent is used to detect for the presence of cDNA in our samples using qPCR...
The LabAssay™ Series is a collection of biochemical assay kits for analyzing samples from ...
Designed for quick turnaround of plasmid, amplicon, or synthetic construct sequencing, ...