RNA, or ribonucleic acid, is not only a predecessor of protein formation, but also a regulator of gene expression. Total RNA, mRNA, or small RNA is very difficult to isolate due to its instability and biologically low quantities. It can be challenging to accumulate an adequate amount of rare sample tissue or cells necessary to carry out a series of experiments. Purchasing purified RNA is simplified with the multitude of suppliers that offer several forms of RNA from multifarious organisms, tissues, and cells. RNA samples are prepared in reagents that are compatible with subsequent isolation protocols for library construction or assays such as quantitative real-time PCR. The analysis of gene expression suppression is feasible using miRNA (microRNA) from adult and fetal tissue sources, and from mouse brain. Obtaining yeast tRNA or feline total RNA from bone marrow in practicable concentrations suitable for diverse applications is quick and simple.
MicroRNAs are small endogenous RNAs that play a crucial role in translational ...
MicroRNAs (miRNA) were first identified in the 1990s and ever since miRNA ...
Thursday, August 11, 2011
Although microarrays are an essential microRNA (miRNA) screening tool for many, another tool with a ...
RNA interference (RNAi) – the process of disrupting gene expression such that the gene’s ...
Dr. Stephen Bustin presents a MIQE-based evaluation of qPCR assay design and publications including qPCR including RNA integrity and purity, primer specificity, PCR and normalization of expression data.
Dr. Mano discovered the lung cancer oncogene EML4K-ALK gene fusion in 2007. In this webinar Dr. Mano reviews his current research using SureSelect RNA capture products to focus his Next Generation Sequencing (NGS) experiments. By focusing on a ...
Saturday, August 20, 2011
Martin Kerick, PhD discusses targeted next generation re-sequencing in clinical settings with a study of microsatellite instability in colon cancer.
Researchers at Penn State University have developed a chemical model that mimics a possible step in the formation of cellular life on Earth four-...
For the last dozen years, scientists have known that minuscule strings of genetic material called small RNA are critically important to our genetic ...
Indiana University biologists have found that specific types of RNA polymerase enzymes, the molecular machines that convert DNA into RNA, can differ ...