GeneSwitch™ Complete Kit
from
Invitrogen
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Product
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GeneSwitch™ Complete Kit
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Company
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Invitrogen
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Price
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Pricing Info
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More Information
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View Company Product Page
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Applications
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Mammalian Expression and Cloning
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Host
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Mammalian
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Promoter
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E1b (GAL4-E1b)
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Quantity
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1 kit
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Resistance
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Zeocin
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Type
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Plasmid
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Vector Name
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pGene/V5-His /LacZ, pGene/V5-His B, pGene/V5-His A, pGene/V5-His C, pSwitch
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Catalog Number
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K106001
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Selectable Marker
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Zeocin
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Description
The GeneSwitch™ System for inducible mammalian expression is ideal for experiments that require the absolute lowest uninduced expression levels. The expression vector pGene/V5-His provides a minimal promoter, GAL4-E1b, consisting of the binding sites for the yeast Gal4 DNA binding protein followed by the TATA sequence from the Adenovirus E1b promoter. Without additional factors, the GAL4-E1b promoter is transcriptionally silent.To activate transcription from the GAL4-E1b promoter, the GeneSwitch™ regulatory protein is expressed from a minimal TK promoter on the pSwitch vector.