pcDNA™5/FRT/TO TOPO TA Expression Kit
from
Invitrogen
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Product
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pcDNA™5/FRT/TO TOPO TA Expression Kit
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Company
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Invitrogen
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Price
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Pricing Info
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More Information
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View Company Product Page
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Applications
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Mammalian Expression and Cloning
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Host
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Mammalian
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Promoter
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CMV, TO
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Quantity
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20 reactions
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Resistance
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ampicillin/ hygromycin
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Type
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Plasmid
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Vector Name
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pcDNA5/FRT/TO-TOPO, pcDNA5/FRT/TO/CAT
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Catalog Number
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K651020
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Selectable Marker
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Hygromycin
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Description
pcDNA™5/FRT/TO, pcDNA5/FRT/TO-TOPO®; and pcDNA™5/FRT/TO-E are designed for targeted Flp-In™ integration followed by tetracycline-regulated expression. Each vector contains a FRT site for efficient targeted integration and a hybrid CMV/TetO2 promoter for regulated expression. In addition, pcDNA5/FRT/TO-TOPO®; and pcDNA™5/FRT/TO-E are adapted for TOPO®;-and Echo™ Cloning, respectively. TOPO®; Cloning enables rapid ligation of PCR products into pcDNA5/FRT/TO-TOPO®; in just five minutes on your bench top. Echo™ Cloning allows you to quickly shuttle your gene of interest into multiple expression vectors.
The Flp-In™ T-REx™ 293 Cell Line is available to save time in expression experiments. This cell line contains pFRT/lacZeo and pcDNA™6/TR stably integrated and is tested for its ability to regulate expression.