TRIMMER-Direct cDNA Normalization Kit
from
Evrogen JSC
Description
cDNA normalization is essential for hunting and analyzing the rare transcripts using high-throughput approaches, such as EST sequencing projects, microarrays, functional screenings etc. The Evrogen TRIMMER-DIRECT kit is developed for normalization of cDNA enriched with full-length sequences and intended for directional cloning. While most normalization methods are not well suited for the normalization of long cDNA, TRIMMER-DIRECT allows to obtain normalized cDNA samples in which individual cDNA sizes range from 0.5 to 4.5 kb. The normalization is based on unique properties of novel Duplex Specific Nuclease (Zhulidov et al., Nucl. Acids. Res. 2004, 32: e37). It is performed prior to cDNA cloning, and does not include physical separation steps. Normalization using the TRIMMER-DIRECT kit significantly increases the efficiency of random sequencing of amplified cDNA.