Chromatin Immuno-precipitation Made Easy

July 14, 2014

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University of Delhi
Human Genetics
Ph.D. student

Company:

Diagenode

Product Name:

SX-8G IP-Star Automated System

Catalog Number:

B03000001 (UH-001-0001)

Our research lab is a HIV lab and we study how HIV affects the immune system and takes over the host cells. We use Jurkat cells and mouse thymocytes as model systems as well as patient samples. Performing a ChIp with patient samples can be a tough task. And if you perform ChIp in batches, the results may vary. That's when our lab bought an IPStar automated ChIp workstation.

Experimental Design and Results Summary

Tips

Simulate the whole program and check it twice. Smallest mistake can ruin the whole experiment and cost a lot of money.

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Summary

The Good

Total automation, computer controlled, simple software

The Bad

Expensive reagents (Diagenode advises to buy everything from them, even antibodies), machine is quite large in size, software is a bit confusing, expensive

The Bottom Line

A very good instrument if you are handling multiple ChIP samples and if you are concerned about the variations in different samples.

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