Novus Biologicals
KMT2A/MLL Antibody
NB600-256
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Browse Similar Products: KMT2A/MLL Western Blot Antibodies
Our lab works on enhancer-promoter interactions in cancer cells. I used MLL1 antibody to check the levels of this protein, a histone H3 lysine 4 methyltransfarese in cancer cells. H3K4me(1) is an important mark of enhancers.
Western Blot
HL-60 and HEK-293T whole cell lysates
1:1000, at 4 degree O/N in TBST on a rocker
5% BSA in TBST for one hr
Goat anti-rabbit IgG HRP in 5% BSA at room temperature for 1 hr
N/A
ECL for 15 sec exposure with x-ray film
This antibody detects the specific band but also gives many other bands, which is a common case with most MLL antibodies.
None
Gives a strong signal
Multiple bands
Despite multiple bands, I would say it is a good antibody to detect MLL1