Life Technologies
Total Exosome Isolation Reagent (from serum)
4478360
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Our research focuses on microRNAs, especially in how they can be used in medical applications.
I have used the Life technologies Exosome Isolation reagent to isolate exosomes from human serum. These were then used to extract RNA and quantitate microRNA expression by qRT-PCR.
Measurement of microRNA expression from serum exosomes
250ul aliquots of human serum samples
The protocol is very simple with minimal hands-on time. Briefly, the samples are thawed and then centrifuged at 2000xg for 30 minutes to remove cells and debris. The supernatant is then transferred to a new tube and 0.2 volumes of the Exosome Isolation Reagent added. This is incubated at 2-8C for 30 minutes and then centrifuged at 10,000xg for 10 minutes. Finally, the supernatant is removed leaving the exosome pellet which can be further analysed in downstream applications.
This product is more competitively priced than EqoQuick
Excellent levels of isolation as assessed by electron microscopy. RNA isolated from the exosomes suitable for downstream analysis by qRT-PCR.
None
Simple methodology for effective isolation. Good price.
Requires 30 minutes of incubation plus a 30 minute centrifugation which is time consuming.
Good value product for effective exosome isolation.
Product Name: Total Exosome Isolation Reagent (from serum)Supplier Page