Sec8 Antibody

Overall

Quality of Results

Ease-of-Optimization

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Biology
University of Pennsylvania
Graduate student

Company:

Enzo Life Sciences

Product Name:

rSec8 mAb (14G1)

Catalog Number:

ADI-VAM-SV016-F

Our lab is studying a multi-protein complex named exocyst. Sec8 is one of the eight subunits of the exocyst complex. We use this antibody for western blot, IF and IP to detect Sec8 expression level, localization and interaction with other proteins. 

Sec8 antibody is used to detect endogenous Sec8 proteins in cell lysates or coimmunoprecipitated by known binding partners.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Hela, 293T cell lysates

Primary Incubation

1:2000; 4 degree overnight

Blocking Agent

5% milk in TBST

Secondary Incubation

HRP conjugated anti-mouse antibody

Tertiary Incubation

N/A

Detection

ECL

Results Summary

Sharp and clean bands at correct molecular weight

Additional Notes

None

Related Categories

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Summary

The Good

Sensitive and clean

The Bad

N/A

The Bottom Line

Excellent antibody!

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