S100A4 Detected by IHC

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review
Cancer Research Program
Fundación IMIM
Lab manager

Company:

Millipore

Product Name:

Anti-FSP1/S100A4 Antibody

Catalog Number:

07-2274

S100A4 is highly expressed in fibroblasts after activation of the cell. Stroma of the tumors, inflamation or injuries are cellular processes which could activate and recruit fibroblasts. We use this marker to detect those active fibroblasts in tumoral samples to analyze patient or mice tumoral samples.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Mice tumors

Primary Incubation

1/500

Blocking Agent

1%FBS 1%BSA in TBS-T

Secondary Incubation

Leyca Powervision

Tertiary Incubation

Not needed

Detection

DAB

Results Summary

Good nuclear detection in stromal fibroblast.

Additional Notes

Heat mediated retrieval needed (citrate pH6).

Related Categories

Image Gallery

Summary

The Good

Strong signal

The Bad

Any remarkable.

The Bottom Line

Nice antibody to detect activated fibroblasts. Some other cells are stained.

Share your experience with other scientists. Write a Review! »

Join the discussion