Excellent Antibody

Dept Imaging Sciences 4th floor, Lambeth Wing, King's College London, St Thomas' Hospital
King's College London
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

APC anti-human CD132 (common γ chain) Antibody

Catalog Number:

338608

Clone Number:

TUGh4

I am using this antibody to determine expression levels, and any changes in expression of the trimeric IL-2 receptor on activated human T-cells and on tumour cells. I am using this antibody as part of the panel to look at all three proteins that make up the high affinity receptor

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human T-cells and tumour cells

Primary Incubation

1uL/million cells, 15mins, room temperate

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometry

Results Summary

This product gives great MFI for positive samples with very low background staining. Because of this, it gives great separation between positive and negative samples and makes analysis really clean and easy

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very accessible and easy to use. Gives great separation between positive and negative populations for easy analysis. Great MFI

The Bad

None

The Bottom Line

Great antibody to use and a little goes a long way

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