Biolegend
PE/Cyanine7 anti-mouse CD49b Antibody
103517
HMα2
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It is well known that B lineage cells play a major role in pathogenesis of autoimmune diseases through presentation of antigen to T cells, production of autoantibodies and inflammatory cytokines that modulate immune responses. We are interested in elucidating the biology of B lineage cells during autoimmunity. In this experiment, I wanted to titrate anti-CD49b
Flow Cytometry
Wild-type mouse spleen
Surface staining with, CD49b PE-Cy7 (1/800) CD19, etc. (20 min, 4° in the dark)
Clone 2.4G2 (Fc block)
N/A
Flow cytometry, yelllow green laser
I could stain CD49b cells in the spleen using the antibody at 1/800. The plots presented are gated in live CD4-CD19- cells.
The antibody works well, it is an excellent option and works at relatively high dilution.
None
It can be used advantageously