Great Antibody to Detect Perforin Expression in T Cells

December 14, 2020

Immunobiology
Yale University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Biolegend

Product Name:

Anti-Mouse Perforin APC

Catalog Number:

154304

Clone Number:

S16009A

Perforin is a critical effector molecule expressed in CD8+ T cells to eliminate target cells. This antibody detects perforin levels in T cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Tumor lymphocytes

Primary Incubation

1:500

Blocking Agent

FC Block in FACS buffer during surface staining

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Cytek Aurora (3 laser, BVR)

Results Summary

We can easily identify perforin positive CD8+ T cells in the tumor.

DOI or PMID #

N/A

Additional Notes

This antibody is part of the intracellular staining protocol step.

Related Categories

Image Gallery

Summary

The Good

Robustly identifies perforin in CD8 T cells in the tumor.

The Bad

None

The Bottom Line

Great reliable antibody to detect perforin expression via flow cytometry.

Share your experience with other scientists. Write a Review! »

Join the discussion