A Good Anti mouse TNFalpha Antibody

December 08, 2020

Innate Immunity
DRFZ
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Brilliant Violet 421™ anti-mouse TNF-α Antibody

Catalog Number:

506327

Clone Number:

MP6-XT22

This antibody was used in order to detect TNFalpha producing T cells isolated from murine spleen

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine spleen

Primary Incubation

15' 4oC

Blocking Agent

0.5% BSA in PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACSymphony, software FACSDiva

Results Summary

This antibody stained TNFalpha producing (CD4+ and CD4-) T cells derived from mouse spleen with high specificity and resolution

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Specific, high resolution

The Bad

Nothing

The Bottom Line

I would recommend this antibody for detection of cells producing TNFalpha

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