Miltenyi
CD49d Antibody, anti-mouse, PE-Vio 770, REAfinity
130-111-979
Clone REA807
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It is well known that B lineage cells play a major role in pathogenesis of autoimmune diseases through presentation of antigen to T cells, production of autoantibodies and inflammatory cytokines that modulate immune responses. We are interested in elucidating the biology of B lineage cells during autoimmunity. In this experiment, I wanted to stain CD49d B cells.
Flow Cytometry
Wild-type mouse spleen
Surface staining with CD49d PE-Vio 770 (1/800) CD19, etc. (20 min, 4° in the dark)
Clone 2.4G2 (Fc block)
N/A
Flow cytometry, yelllow green laser
I could stain CD49d cells in the spleen using the antibody at 1/800. The histograms presented are gated in live CD19+ cells.
The antibody works well, and it is a recombinant antibody, animal-free
None
It can be used.