Great Antibody for Western Blot

December 08, 2020

BlueskyReddit
Medicine
Harvard Medical School
Post-doctoral fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technologies

Product Name:

Lamin B2 (D8P3U) Rabbit mAb

Catalog Number:

12255S

Clone Number:

N/A

I used the Lamin B2 antibody as a nuclear marker for nuclear protein faction analysis.

Experimental Design and Results Summary

Applications

Western Blot

Sample

THP-1 cells treated with different compounds

Primary Incubation

1:1000 dilution, 18 hours at 4 degrees with gentle shaking

Blocking Agent

5% non-fat dry milk in Tris buffer containing 0.1% Tween-20

Secondary Incubation

HRP-conjugated anti-rabbit antibody 1:2000 dilution, 1 hour at room temperature with gentle shaking

Tertiary Incubation

Not applicable

Detection

ECL based detection

Results Summary

This antibody specifically detects lamin B2 protein present in the nuclear fraction.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

high specificity and no background

The Bad

Nothing

The Bottom Line

It is a great antibody for Western Blotting.

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