Measuring Expression of LPAR3 in Microglia Cells

December 10, 2020

Molecular Biology and Biochemistry
Medical University of Graz
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Cayman Chemical

Product Name:

LPA3 Polyclonal Antibody

Catalog Number:

10004840

Clone Number:

N/A

BV2 microglia cells were treated with LPS or IL4 and changes in the expression of all LPA receptors were accessed by western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

BV2 microglia cells

Primary Incubation

1:400 overnight at +4

Blocking Agent

5% milk

Secondary Incubation

1:5000 goat anti-rabbit, 2 hours at room temperature

Tertiary Incubation

N/A

Detection

ChemiDoc System

Results Summary

IL4 treatment induced high expression of LPAR3 in microglia cells. LPAR3 appeared to not be expressed under control conditions or after LPS treatment.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very good signal

The Bad

N/A

The Bottom Line

Highly recommended

Share your experience with other scientists. Write a Review! »

Join the discussion