PE-Cy™7 Rat Anti-Mouse CD107a

December 10, 2020

BlueskyReddit
Innate Immunity
DRFZ
Graduate Student

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Company:

BD Biosciences

Product Name:

PE-Cy™7 Rat Anti-Mouse CD107a

Catalog Number:

560647

Clone Number:

1D4B

Info: Supplier Page 

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We used this antibody in order to mark degranulation in Natural Killer cells after stimulation of murine splenocytes with PMA and Ionomycin

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine spleen

Primary Incubation

The antibody was added during the stimulation for 4h

Blocking Agent

RPMI with 10% FCS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACSymphony, software FACSDiva

Results Summary

This antibody was added in the culture of murine splenocytes for 4h during PMA/Iono Stimulation. It succesfully marked the degranulating Natural Killer cells with high specificity and resolution.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Specific, high resolution

The Bad

Nothing

The Bottom Line

I would recommend this antibody

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