Abcam
Anti-53BP1 antibody
ab36823
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Ionizing radiation is thought to produce many of its damaging effects on cells by producing clustered DNA double-strand breaks (DSB), promoting hyperphosphorylation of DSB-associated proteins, and inducing formation of discrete nuclear foci containing p53-binding protein 1 (53BP1), an important regulator of DSB signaling that is regulated by γ-radiation.
Immunofluorescence
Caco2 cells
1:100, overnight 4C
Donkey serum
1:500 secondary, 1 h, room temp
N/A
Fluorescence or confocal microscopy
53BP1 was diffusely localized in the nuclei of control group cells, however it relocated to punctate nuclear foci within cells when they were irradiated.
Very specific
Reproducible results, highly recommend it