Anti-GAPDH antibodies are used in the immunodetection of the protein glyceraldehyde-3-phosphate dehydrogenase. In humans, the canonical protein has a reported length of 335 amino acid residues and a mass of 36.1 kDa. Its subcellular localization is in the nucleus and cytoplasm. Up to 2 different isoforms have been reported for this protein. It is notably widely expressed in many tissue types. A member of the Glyceraldehyde-3-phosphate dehydrogenase protein family, GAPDH is known to have both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Synonyms for this target antigen include GAPD, HEL-S-162eP, OCAS, p38 component, Oct1 coactivator in S phase, 38 Kd component, and G3PD. GAPDH gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. Over 10000 citations in the literature describe the use of GAPDH antibodies in research. Western Blot is a widely used application for these antibodies. ELISA, Immunofluorescence, and Immunohistochemistry are also common applications.