Abcam
Anti-GAPDH antibody [6C5]
ab8245
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Browse Similar Products: GAPDH Western blot Antibodies
GAPDH is considered as a house keeping gene and so its levels are used as a metric to determine the loading efficiency in gels (unless the experiment affects GAPDH levels. If yes, then an alternative loading control should be done).
Western Blot
Whole cell extracts of NIH-3T3 cells_50 µg loaded per lane
1:2000 dilution in blocking buffer. Incubation at 4 deg for 16 hr on a rotator
5% Milk in 1x TBS-T (0.1%)
1:5000 dilution in blocking buffer. Incubation at 25 deg for 1 hr on a rotator
NA
ECL_2 sec exposure on a Film
Excellent antibody for Western Blotting. The dilution can be increased higher to 1:5000-1:10000
None
High signal to noise ratio.
Excellent antibody for Western Blotting