Good GAPDH Antibody for Western Blot

October 09, 2018

BlueskyReddit
School of Graduate Studies
SUNY Downstate Medical Center
Graduate Student

Overall

Quality of Results

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Company:

Cell Signaling Technologies

Product Name:

GAPDH (D16H11) XP® Rabbit mAb #5174

Catalog Number:

5174T

Our aim was to determine changes in methylation pattern of H3K9me3 by changing Setdb1 expression in PC3 cells. For this study, we knocked down Setdb1, and GAPDH knockdown was used as a positive control, which was detected using western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human cell lines PC3 lysates

Primary Incubation

Overnight, 4 degree C, 1:1000

Blocking Agent

5% milk

Secondary Incubation

Goat anti rabbit

Tertiary Incubation

N/A

Detection

Hrp-dab reaction

Results Summary

GAPDH was completely knocked down in the positive control added with GAPDH siRNA but not in cells incubated with non-targeting siRNA or Setdb1 siRNA.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very sepcific

The Bad

None

The Bottom Line

Good control antibody for western blots.

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