Santa Cruz Biotechnology
GAPDH Antibody (G-9)
sc-365062
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We tested different antibodies that work for loading control of GAPDH and we found sc-365062 works well in our hands for both mouse and human tissues and cell lysates
Western Blot
Cell lysates from human fibroblasts
1:1000 in 5% milk
5% Milk for 1 hr
1:10,000 dilution anti-mouse conjugated with HRP secondary antibody
NA
ECL based detection
Best loading control GAPDH antibody for both mouse and human tissues and cell lysates
None
Works well both for human and mouse samples
None noted
Good GAPDH antibody that can be used for both human and mouse samples.