Good GAPDH Western Blot Antibody from GeneTex

November 18, 2019

Department of Pharmacology and Toxicology
Augusta University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

GeneTex

Product Name:

GAPDH

Catalog Number:

GTX627408

Clone Number:

GT239

DCA is an inhibitor of Pdk and enhances the activity of Pdh, resulting in increased turnover of pyruvate to acetyl CoA thereby augmenting oxidative phosphorylation.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Rat Tissue lysate samples

Primary Incubation

1:1000 dilution in 5% skim milk prepared in 1X TBST

Blocking Agent

5% skim milk prepared in 1X TBST

Secondary Incubation

1:4000 5% skim milk prepared in 1X TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

We found time dependent increase of GAPDH by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

No background and very strong signal

The Bad

None

The Bottom Line

This antibody is very specific and the signal is strong. Highly recommended.

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