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Anti-GAPDH antibody [6C5] - Loading Control
Anti-GAPDH antibody [6C5] - Loading Control from Abcam
(7)
Write a review
Citations:
(>1k)
Supplier Page
Supplier Page from
Abcam for
Anti-GAPDH antibody [6C5] - Loading Control
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-GAPDH antibody [6C5] - Loading Control
Company
Abcam
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
ab8245
Antibody Type
Primary
Clonality
Monoclonal
Applications
Immunocytochemistry/ Immunofluorescence, Western blot
Host
Mouse, Nude mouse
Quantity
100 UG
Clone
6C5
Isotype
IgG1
Conjugate/Tag
Unconjugated
Format
Purified by chromatography on protein A Sepharose. Purity is tested by electrophoresis
Concentration
2 mg/ml
Gene Name
GAPDH
Reactivity
Human, Mouse, Rat
Target
GAPDH
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (3825)
(1)
LncRNA TUG1 promotes pulmonary fibrosis progression via up-regulating CDC27 and activating PI3K/Akt/mTOR pathway
Epigenetics
December 31, 2023
Fei Qi, Zhong-Dong Lv, Wen-Di Huang, Shan-Chen Wei, Xin-Min Liu, Wei-Dong Song
(
AP0094
,
1:500
,
ABclonal
), mTOR (
A2445
,
1:500
,
ABclonal
),
CDC27
(
A3333
,
1:1000
,
ABclonal
), and GAPDH (
ab8245
,
1:2000
,
Abcam
) at 4°C overnight, followed by
incubation
with secondary antibody (bs-0295 G-HRP,
1:1000
,
(2)
MiR-18a-5p attenuates HER2-positive breast cancer development by regulating PI3K/AKT pathway
Cancer Biology & Therapy
December 31, 2023
Yongjun Liu, Hua Yang
(
ab8805
,
Abcam
), P-PI3K (ab278545, Abcam), PI3K (
ab32089
,
Abcam
, UK),
HER2
(
ab134182
,
Abcam
), and GAPDH (
ab8245
,
Abcam
) were incubated with the
membranes
after blocking at 37°C for an hour with 5% nonfat milk. The
(3)
Gallic acid improves the metformin effects on diabetic kidney disease in mice
Renal Failure
December 31, 2023
Yan Hong, Jidong Wang, Wenjuan Sun, Lai Zhang, Xuefang Xu, Kaiyue Zhang
EPR19549] to AMPK alpha 1 + AMPK alpha 2,
1:1000
), anti-
SIRT1
(#
ab110304
, 1.25 µg/mL), and anti-GAPDH (#
ab8245
,
1:1000
) from
Abcam
. Protein signals were detected using enhanced chemiluminescence (ECL). The DHE
(4)
HYAL1 deficiency attenuates lipopolysaccharide-triggered renal injury and endothelial glycocalyx breakdown in septic AKI in mice
Renal failure
December 1, 2023
Xing H, Li S, Fu Y, Wan X, Zhou A, Cao F, Sun Q, Hu N, Ma M, Li W,
et al
.
anti-LC3-I (ab62721;
1:1000
), anti-LC3-II (ab192890;
1:2000
), anti-p62 (ab109012;
1:10000
) and GAPDH (
ab8245
;
1:500
) at 4 °C overnight and subsequently with
horseradish
peroxidase-labeled IgG (
ab6721
;
1:1000
)
(5)
DHX9 Strengthens Atherosclerosis Progression By Promoting Inflammation in Macrophages
Inflammation
October 1, 2023
Huangfu N, Ma H, Tian M, Zhang J, Wang Y, Li Z, Chen X, Cui H.
Proteintech
),
p65
(No.
66535–1-Ig
,
Proteintech
),
p65
(phospho S536) (p-
p65
,
sc-136548
,
Santa Cruz
), GAPDH (
ab8245
,
Abcam
), flag (
ab205606
, Proteintech), or Lamin B1 (No.
66095–1-Ig
,
Proteintech
). After incubating with
(6)
Kappa opioid receptor in nucleus accumbens regulates depressive-like behaviors following prolonged morphine withdrawal in mice
iScience
September 15, 2023
Jinyu Zhang, Ye Lu, Min Jia, Yuying Bai, Lulu Sun, Ziqing Dong, Wenrong Tian, Fangyuan Yin, Shuguang Wei, Yunpeng Wang
anti-Synaptophysin,Servicebio,Cat# GB11553; RRID: AB_2937063 Mouse monoclonal anti-GAPDH,
Abcam
,Cat#
ab8245
; RRID:AB_2107448 Cy3 Goat Anti-Mouse IgG,Abclonal,Cat#AS008; RRID:AB_2769088 Fluorescein Anti-Avidin
(7)
circAGTPBP1 promotes the progression of papillary thyroid cancer through the notch pathway via the miR-34a-5p/notch1 axis
iScience
September 15, 2023
Dai L, Zhang W, Wang Y, Yu K, Le Q, Wu X.
Abcam
), anti-Hes1 (cat. #
ab71559
,
Abcam
),
anti-Jagged1
(cat. #ab300561, Abcam), and anti-GAPDH (cat. #
ab8245
,
Abcam
) overnight at 4°C. Then, the
membranes
were incubated with secondary antibody (Abcam) for 2 h.
(8)
Human genetic adaptation related to cellular zinc homeostasis
PLoS genetics
September 1, 2023
Roca-Umbert A, Garcia-Calleja J, Vogel-González M, Fierro-Villegas A, Ill-Raga G, Herrera-Fernández V, Bosnjak A, Muntané G, Gutiérrez E, Campelo F,
et al
.
diluted in the corresponding blocking solution anti-HA for
ZnT9
(
H3663
,
Sigma
-Merck), and anti-GAPDH (
ab8245
,
Abcam
). HRP secondary antibodies (
1:1000
; GE Healthcare) were used depending on the primary antibody.
(9)
S100A5 Attenuates Efficiency of Anti-PD-L1/PD-1 Immunotherapy by Inhibiting CD8(+) T Cell-Mediated Anti-Cancer Immunity in Bladder Carcinoma
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
September 1, 2023
Li H, Chen J, Li Z, Chen M, Ou Z, Mo M, Wang R, Tong S, Liu P, Cai Z,
et al
.
overnight with primary antibodies: anti‐
S100A5
(Cat:
17924‐1‐AP
,
Proteintech
, USA) and anti‐GAPDH (Cat:
ab8245
,
Abcam
, USA). After washing with TBST for three times, the PVDF
membrane
was incubated at room temperature
(10)
Site-specific phosphorylations of the Arf activator GBF1 differentially regulate GBF1 function in Golgi homeostasis and secretion versus cytokinesis
Sci Rep
August 21, 2023
Kendall Walton, Tomasz J. Nawara, Allyson R. Angermeier, Hadley Rosengrant, Eunjoo Lee, Bridge Wynn, Ekaterina Victorova, George Belov, Elizabeth Sztul
secondary antibodies were used at a
1:5000
concentration. Antibodies Mouse monoclonal anti-GAPDH (
ab8245
) and rabbit polyclonal anti-GFP (
ab290
) were purchased from
Abcam
(Cambridge, MA).
Mouse
monoclonal
(11)
MIF is a critical regulator of mononuclear phagocytic infiltration in hepatocellular carcinoma
iScience
August 18, 2023
Yunxi Liao, Chenyang Wu, Yang Li, Jinhua Wen, Dongyu Zhao
RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Anti-
SPP1
,
Abcam
,Cat#
ab214050
Anti-
MIF
,
Abcam
,Cat#
ab187064
Anti-GADPH,
Abcam
,Cat#
ab8245
HRP-labeled Goat Anti-Rabbit IgG (H+L),Beyotime,Cat# A0208 ,, Bacterial and
virus
strains
,Bacterial
(12)
Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1
iScience
August 18, 2023
Tong L, Rao J, Yang C, Xu J, Lu Y, Zhang Y, Cang X, Xie S, Mao J, Jiang P.
RRID:AB_2915918
NGAL
,
Proteintech
,Cat#
26991-1-AP
; RRID:AB_2880715
XPNPEP3
,
Genetex
,Cat#
GTX105541
; RRID:AB_1952597 GAPDH,
Abcam
,Cat#
ab8245
; RRID:AB_2107448
TOM20
,
Abclonal
,Cat#
A19403
; RRID:AB_2862646 UPF1,
Proteintech
,Cat#
23379-1-AP
; RRID:AB_11232421
DCP2
,
Abclonal
,Cat#
A8282
;
(13)
Unraveling the Role of EV-Derived miR-150-5p in Prostate Cancer Metastasis and Its Association with High-Grade Gleason Scores: Implications for Diagnosis
Cancers (Basel)
August 17, 2023
Marian Cruz-Burgos, Sergio A. Cortés-Ramírez, Alberto Losada-García, Miguel Morales-Pacheco, Eduardo Martínez-Martínez, Jorge Gustavo Morales-Montor, Alejandro Servín-Haddad, J. Samuel Izquierdo-Luna, Griselda Rodríguez-Martínez, María Del Pilar Ramos-Godínez,
et al.
(
Thermo Fisher Scientific
, Inc., cat.
10628D
), anti-TSG101 (Abcam, Cambridge, UK, cat. cab83), anti-GAPDH (
Abcam
, cat.
ab8245
), anti-β-actin (
Abcam
, cat.
ab8221
), anti-rabbit HRP secondary (
Abcam
, cat.
ab97051
), and anti-mouse
(14)
Rac GTPase activating protein 1 promotes the glioma growth by regulating the expression of MCM3
Transl Oncol
August 16, 2023
Bo Jia, Yuran Jiang, Yu Huan, Yu Han, Wei Liu, Xiao Liu, Yingwen Wang, Lei He, Zhengcong Cao, Xin He,
et al.
13739-1-AP
and
Abcam
, cat. no.
ab97315
),
GAPDH
(
Proteintech
, cat no.
60004-1-Ig
and
Abcam
, cat. no.
ab8245
);
MCM3
(Proteintech, cat no.
15597-1-AP
); YY1 (
Proteintech
, cat no. 66,281–1-Ig), goat anti-mouse IgG
(15)
PARP1-catalyzed PARylation of YY1 mediates endoplasmic reticulum stress in granulosa cells to determine primordial follicle activation
Cell Death Dis
August 15, 2023
Wei Chen, Qiukai E, Bo Sun, Pengxue Zhang, Nan Li, Shujia Fei, Yingnan Wang, Shuting Liu, Xiaoqiu Liu, Xuesen Zhang
anti-HA-tag (
1:1000
, #30701ES60, YEASEN), anti-FLAG-tag (
1:1000
, #30501ES60, YEASEN) and anti-GAPDH (
1:4000
, #
ab8245
,
Abcam
, Cambridge, UK) antibodies overnight at 4 °C. After TBST washing, the
membranes
were treated
(16)
Programming conformational cooperativity to regulate allosteric protein-oligonucleotide signal transduction
Nat Commun
August 14, 2023
Yuan Liang, Yunkai Qie, Jing Yang, Ranfeng Wu, Shuang Cui, Yuliang Zhao, Greg J. Anderson, Guangjun Nie, Suping Li, Cheng Zhang
penicillin/streptomycin (Sigma-Aldrich). Primary antibodies against
PLK1
(EPR19534(
ab189139
)) and GAPDH (6C5(
ab8245
)) were purchased from
Abcam
(Cambridge, UK). HRP-linked
goat
anti-rabbit secondary antibodies (PR30011)
(17)
A Comparative Study on the Lysosomal Cation Channel TMEM175 Using Automated Whole-Cell Patch-Clamp, Lysosomal Patch-Clamp, and Solid Supported Membrane-Based Electrophysiology: Functional Characterization and High-Throughput Screening Assay Development
Int J Mol Sci
August 14, 2023
Andre Bazzone, Maria Barthmes, Cecilia George, Nina Brinkwirth, Rocco Zerlotti, Valentin Prinz, Kim Cole, Søren Friis, Alexander Dickson, Simon Rice,
et al.
for 24 h: rabbit
anti-TMEM175
(
Proteintech
19925-1-AP
, dilution
1:1000
) and mouse anti-GAPDH (
Abcam
ab8245
, dilution:
1:5000
). Subsequently, the secondary polyclonal antibody Goat anti-rabbit (
Li-Cor
926-32211
,
(18)
ZNF143 inhibits hepatocyte mitophagy and promotes non-alcoholic fatty liver disease by targeting increased lncRNA NEAT1 expression to activate ROCK2 pathway
Epigenetics
August 11, 2023
Yujie Dong, Minjie Hu, Kewei Tan, Rongjuan Dai
followed by detecting the signals with enhanced chemiluminescence reagent (EMD Millipore). GAPDH (
1:10000
,
ab8245
,
Abcam
) used as a control. Statistical analysis Data in this study was presented as mean ± standard
(19)
A mannitol-modified emodin nano-drug restores the intestinal barrier function and alleviates inflammation in a mouse model of DSS-induced ulcerative colitis
Chin Med
August 11, 2023
Yin-Yue Xu, Min Zhu, Jiang Wu, Long-Biao Luo, Si-Jing Dong, Meng-Gai Zhang, Xue Liu, Ke Wang, Hua Luo, Wang-Hui Jing,
et al.
ZO-1
(
1:1000
, Cat #
ab276131
;
Abcam
), Occludin (
1:2000
, Cat #
ab216327
;
Abcam
), and GAPDH (
1:5000
, Cat #
ab8245
;
Abcam
) at 4 °C. The following day, the
membranes
were incubated with secondary antibodies and developed
(20)
N6-methyladenosine (m6A) methyltransferase WTAP-mediated miR-92b-5p accelerates osteoarthritis progression
Cell Commun Signal
August 10, 2023
Zhaowei Lin, Tao Jiang, Wei Zheng, Jiayuan Zhang, Anan Li, Chao Lu, Wengang Liu
1:1000
,
Abcam
), anti-Aggrecan (
ab3778
,
1:750
,
Abcam
), anti-TIMP4 (
ab58425
,
1:750
,
Abcam
), and anti-GADPH (
ab8245
,
1:1000
,
Abcam
). Total RNA m6A quantification Total RNA was separated using TRIzol reagent (Takara,
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Reviews
Write a Review
(1)
12/8/2023
Good GAPDH Antibody From Abcam for Western Blotting
Dr. David Sunnen
The specific goal of this western blot experiment was to evaluate the expression level of protein of interest relative to GAPDH protein expression in cell lysate samples. This antibody worked for ...
Read Review
(1)
8/7/2023
Best GAPDH Antibody From Abcam
Shivali Rawat
I use GAPDH antibody as loading control for total cellular protein fractions or soluble protein fractions regularly under various stress conditions.
(1)
9/13/2021
Great GAPDH Antibody
Jian Chen
GAPDH is a common loading control used for most western blots. This antibody has been working great for most of the cell lines we had worked at.
(1)
8/18/2016
Decent Loading Control Antibody
Shikha Saini
Using a loading control is extremely important for any conclusive experiment. Mostly, any gene which is vital for the cell or is abundantly present can be used as a loading control. GAPDH is one of ...
Read Review
(1)
2/1/2016
GAPDH Antibody for Western Blotting
Prudhvi Pusapati
I am interested in studying the role of various transcription factors during regenerative processes of the lung and intestine after chemical induced tissue injury. The level of transcription factors ...
Read Review
(1)
1/11/2016
Western Blotting GAPDH Antibody from Abcam
Ganesh Pusapati
GAPDH is considered like a house keeping gene and so its levels are used as a metric to determine the loading efficiency in gels (unless the experiment affects GAPDH levels. If yes, then an ...
Read Review
(1)
7/15/2015
Review of Anti-GAPDH antibody
Liyong Z.
To perform Western Blot analysis, the cells were washed twice in PBS and harvested with lysis buffer (LB: 20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1 mM Na2EDTA, 1 mM EGTA, 1% Triton X-100, 2.5 mM sodium ...
Read Review
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Anti-GAPDH antibody [6C5] - Loading Control from Abcam
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
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