Cell Signaling Technology
GAPDH Ab
5014
14C10
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Browse Similar Products: GAPDH Western Blot Antibodies
Loading control antibodies are crucial when assessing the expression level of proteins through WB. Here, I utilized the GAPDH antibody as a loading control in acute myeloid leukemia cell line OCI AML-2 to determine if I generated a KO of my protein of interest (USP22).
Western Blot
AML2 whole cell lysate
1:3000 RT 1hr in 5% skim milk
5% milk - 10 min room temperature
5% milk - 30 min room temperature secondary Ab 1:10K
N/A
ECL - film
Good antibody for western blot with no background. Approx. size 38 kDa. Conditions represent samples following CRISPR/Cas9 mediated deletion of USP22. sgLacZ represents non-targeting control. GAPDH is shown as the loading control.
Works well even at 1:3K dilution.
Good loading control Ab for WB