GAPDH Antibody 2275-PC-100 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemGAPDH Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $439.00Supplier Page View Company Product Page
Catalog Number2275-PC-100
Quantity100 uL
IsotypeIgG
HostRabbit
ApplicationsImmunoprecipitation,Western Blot,Simple Western,Western Blot Control
TargetGAPDH
Conjugate/TagUnconjugated
ReactivityCanine,Human,Mouse,Rat,Xenograft
ClonalityPolyclonal
FormatSupplied as a solution containg PBS
ImmunogenSynthetic peptide corresponding to a portion of the human GAPDH/G3PDH sequence
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
NCBI Full Gene Nameglyceraldehyde-3-phosphate dehydrogenase
NCBI Gene AliasesG3PD, GAPD, HEL-S-162eP
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 6 months, -20 to -70 °C under sterile conditions after opening. <span style="font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", system-ui, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Web", sans-serif; font-size: 14px; white-space-collapse: preserve;">

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human, mouse, and rat GAPDH/G3PDH in Western blots.

Sizes Available: 100 uL

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Sodium Channel Isoform Diversity Underlies Chamber-Specific Cardiac ExcitabilityCirculation ResearchApril 24, 2026Colin J. Clark, Christian E. Anderson, Alex Dou, Jason M. Dierdorff, Jason D. Galpin, Lionel Gissot, Samantha G. Thompson, Hannah Choi, Jin-Young Yoon, Daniel T. Infield, et al.primary polyclonal antibody for NaV1.5 (Alomone Labs; Cat. No. ASC-005). GAPDH (R&D Systems, Cat. No. 2275-PC-100) and Na+/K+ ATPase (Proteintech, Cat. No. 55187–1-AP, 1:2000) were used as loading controls for whole

(2) From bats to humans: uncovering ISG15 as a new resistance factor in type 1 diabetesbioRxivApril 6, 2026Eugenia Martin-Vazquez, Xiaoyan Yi, Maressa Fernandes Bonfim, Sayro Jawurek, Priscila L. Zimath, Arturo Roca-Rivada, Junior Garcia Oliveira, Jose Maria Costa-Junior, François Pattou, Julie Kerr-Conte, et al.Proteintech, USP18 (D4E7) Rabbit mAb #4813 Cell Signaling, Human GAPDH Antibody (Bio-Techne Sales Corp. 2275-PC-100) or Anti-TUBA4A (TUBA1) Antibody #T5168 Sigma diluted 1:1,000 in TBST (TBS, 0.1% Tween 20) with 5% BSA.

(3) Sodium Channel Isoform Diversity Underlies Chamber-Specific Cardiac ExcitabilityCirc ResMarch 25, 2026Colin J. Clark, Christian E. Anderson, Alex Dou, Jason M. Dierdorff, Jason D. Galpin, Lionel Gissot, Samantha G. Thompson, Hannah Choi, Jin-Young Yoon, Daniel T. Infield, et al.primary polyclonal antibody for NaV1.5 (Alomone Labs; Cat. No. ASC-005). GAPDH (R&D Systems, Cat. No. 2275-PC-100) and Na+/K+ ATPase (Proteintech, Cat. No. 55187–1-AP, 1:2000) were used as loading controls for whole

(4) Enhanced Delivery of Aurora Kinase A Inhibitor Alisertib via Tumor-Targeting Immunoliposome Nanocomplex for Improved Treatment of Cancers Including Atypical Teratoid/Rhabdoid TumorInt J NanomedicineJanuary 8, 2026Sang-Soo Kim, Manish Moghe, Antonina S Rait, Joe B Harford, Esther H Changwas used as an internal control for protein loading, detected using a rabbit polyclonal antibody (#2275-PC-100, 1:1000 dilution; Novus Bio, Centennial, CO, USA). Bands were visualized using Radiance Q chemiluminescent

(5) Enhanced Delivery of Aurora Kinase A Inhibitor Alisertib via Tumor-Targeting Immunoliposome Nanocomplex for Improved Treatment of Cancers Including Atypical Teratoid/Rhabdoid TumorInternational Journal of NanomedicineJanuary 8, 2026Sang-Soo Kim, Manish Moghe, Antonina S Rait, Joe B Harford, Esther H Changwas used as an internal control for protein loading, detected using a rabbit polyclonal antibody (#2275-PC-100, 1:1000 dilution; Novus Bio, Centennial, CO, USA). Bands were visualized using Radiance Q chemiluminescent

(6) Repurposing statins and phenothiazines to treat chemoresistant neuroblastomaEMBO Molecular MedicineDecember 23, 2025Katarzyna Radke, Kristina Aaltonen, Erick A Muciño-Olmos, Javanshir Esfandyari, Aleksandra Adamska, Joachim T Siaw, Dora Adamic, Chiara Lago, Adriana Mañas, Alexandra Seger, et al.Thermo Fisher Scientific), PARP (1:2000; RRID:AB_2160739, #9542, Cell Signaling Technology), GAPDH (1:5000; RRID:AB_210745, #2275-PC-100, R and D Systems) and actin (1:10,000; RRID: AB_2537667, #MA5-15739-HRP, Thermo Fisher Scientific) as

(7) Targeted ferroptosis induction enhances chemotherapy efficacy in chemoresistant neuroblastomanpj Precision OncologySeptember 16, 2025Adriana Mañas, Alexandra Seger, Aleksandra Adamska, Kyriaki Smyrilli, Joachim T. Siaw, Katarzyna Radke, Erick A. Muciño-Olmos, Oscar C. Bedoya-Reina, Javanshir Esfandyari, Kristina Aaltonen, et al.#1704271). Antibodies used were anti-GPX4 (Cell Signaling Technology, #59735) and anti-GAPDH (R&D Systems, #2275-PC-100). Imaging was performed using Luminata Forte Western HRP substrate (Millipore Sigma) and Amersham Imager 600

(8) Response splicing quantitative trait loci in primary human chondrocytes identify putative osteoarthritis risk genesNature communicationsAugust 26, 2025Byun S, Shine J, Coryell P, Kramer NE, D'Costa S, Thulson E, Parkus SM, Chubinskaya S, Loeser RF, Diekman BO, et al.

(9) Differential immune- and apoptosis-related gene signatures in pancreatic alpha and beta cells contribute to their fate in type 1 diabetesbioRxiv : the preprint server for biologyJuly 31, 2025Yi X, Martin-Vazquez E, Jawurek S, Zimath PL, Oliveira JG, Costa-Junior JM, Ilegems E, Wesley JD, Title AC, Yesildag B, et al.(58D6) Rabbit mAb #9167, Stat1 (9H2) Mouse mAb #9176 or Human/Mouse/Rat GAPDH Antibody R&D Systems # 2275-PC-100 diluted 1:1000 in TBST (TBS, 0.1% Tween 20) with 5% BSA (GAPDH was diluted 1:10 000). The densitometric

(10) Non-canonical ALK7 pathways promote pancreatic cancer metastasis through β-catenin/MMP-mediated basement membrane breakdown and intravasationMolecular cancerJuly 4, 2025Kolarzyk AM, Kwon Y, Oh E, Lee KJ, Cho SY, Cano I, Lu R, Kwak TJ, Lee J, Wong G, et al.

(11) Mitochondrial Oxidative Phosphorylation Alterations in Placental Tissues from Early- and Late-Onset PreeclampsiaInternational Journal of Molecular SciencesApril 22, 2025Theresa Lehenauer, Heidi Jaksch-Bogensperger, Sara Huber, Daniel Weghuber, Thorsten Fischer, Johannes A. Mayr, Barbara Kofler, Bettina Neumayer, Daniel Gharehbaghi, Michaela Duggan-Peer, et al.UK; Ab14734), rabbit polyclonal GAPDH (1:1000; 11 h; 4 °C; Novus Biologicals; Centennial, CO, USA; 2275/PC/100), mouse monoclonal anti-NDUFS4 (1:1000; 2 h; room temperature; Abcam; Cambridge, UK; WH0004724M1), mouse

(12) Pyroptotic cell corpses are crowned with F-actin-rich filopodia that engage CLEC9A signaling in incoming dendritic cellsNature immunologyJanuary 1, 2025Holley CL, Monteleone M, Fisch D, Libert AES, Ju RJ, Choi JH, Condon ND, Emming S, Crawford J, Lawrence GMEP, et al.

(13) Y-box binding protein 1/cyclin A1 axis specifically promotes cell cycle progression at G(2)/M phase in ovarian cancerScientific reportsSeptember 17, 2024Murakami Y, Katsuchi D, Matsumoto T, Kanazawa K, Shibata T, Kawahara A, Akiba J, Yanaihara N, Okamoto A, Itamochi H, et al.NB100-59828) were purchased from Novus Biologicals (Centennial, CO, USA). Antibodies against GAPDH (cat. no. 2275-pc100) were purchased from Trevigen (Gaithersburg, MD, USA). Transfection of siRNA RNA-interference assays

(14) SMARCB1 Gene Therapy Using a Novel Tumor-Targeted Nanomedicine Enhances Anti-Cancer Efficacy in a Mouse Model of Atypical Teratoid Rhabdoid TumorsInternational journal of nanomedicineJanuary 1, 2024Kim SS, Moghe M, Rait A, Donaldson K, Harford JB, Chang EH.

(15) Rab32 and Rab38 maintain bone homeostasis by regulating intracellular traffic in osteoclastsCell Structure and FunctionNovember 17, 2023Kanako Tokuda, Shiou-Ling Lu, Zidi Zhang, Yumiko Kato, Siyu Chen, Kazuya Noda, Katsutoshi Hirose, Yu Usami, Narikazu Uzawa, Shinya Murakami, et al.anti-cathepsin K (sc-48353, E-7, Santa Cruz Biochemistry, Dallas, TX, USA): 1/200, and anti-GAPDH (2275-PC-100, R&D System, Minneapolis, MN, USA): 1/5000; secondary antibodies were anti-rabbit IgG HRP (Cell Signaling

(16) Deficiency of endothelial sirtuin1 in mice stimulates skeletal muscle insulin sensitivity by modifying the secretomeNature communicationsSeptember 11, 2023Li Q, Zhang Q, Kim YR, Gaddam RR, Jacobs JS, Bachschmid MM, Younis T, Zhu Z, Zingman L, London B, et al.

(17) Rational engineering of an improved adenosine deaminase 2 enzyme for weaponizing T-cell therapiesImmunooncol TechnolSeptember 1, 2023Cox JR, Jennings M, Lenahan C, Manion M, Courville S, Blazeck J.

(18) v-Src delocalizes Aurora B by suppressing Aurora B kinase activity during monopolar cytokinesisCellular signallingSeptember 1, 2023Ota R, Watanabe T, Wazawa Y, Kuwajima H, Honda T, Soeda S, Saito Y, Yuki R, Fukumoto Y, Yamaguchi N, et al.

(19) Identification of a novel enhancer essential for Satb1 expression in T(H)2 cells and activated ILC2sLife science allianceAugust 1, 2023Nomura A, Kobayashi T, Seo W, Ohno-Oishi M, Kakugawa K, Muroi S, Yoshida H, Endo TA, Moro K, Taniuchi I.TBST (0.05% vol/vol) + 5% (wt/vol) of milk, overnight at 4°C: SATB1 (ab109122; Abcam), GAPDH (REF: 2275-PC-100; R and D systems) and SMC1 (ab9262; Abcam). The blots were then wash three times in 15–30 ml TBST (0.05%

(20) Selective Eradication of Colon Cancer Cells Harboring PI3K and/or MAPK Pathway Mutations in 3D Culture by Combined PI3K/AKT/mTOR Pathway and MEK InhibitionInternational journal of molecular sciencesJanuary 14, 2023Atanasova VS, Riedl A, Strobl M, Flandorfer J, Unterleuthner D, Weindorfer C, Neuhold P, Stang S, Hengstschläger M, Bergmann M, et al.S807/811,-,rabbit,1:1000,#9308,CST IRS1,-,rabbit,1:1000,#2382,CST E-Cadherin,HECD-1,mouse,1:1000,ab1416,Abcam α Tubulin,DM1A,mouse,1:5000,CP06,SigmaAldrich GAPDH,-,rabbit,"1:10,000",2275-PC-100,R&D Systems Ki67,8D5,mouse,1:1000,#9449,CST Cleaved Caspase 3 (CC3),5A1E,rabbit,1:1000,‘9664,CST Primary

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GAPDH Antibody

GAPDH Antibody

GAPDH Antibody

GAPDH Antibody

GAPDH Antibody

GAPDH Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (120)

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GAPDH Antibody from R&D Systems, a Bio-Techne Brand

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