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Anti-GAPDH antibody [6C5] - Loading Control
Anti-GAPDH antibody [6C5] - Loading Control from Abcam
(7)
Write a review
Citations:
(>1k)
Supplier Page
Supplier Page from
Abcam for
Anti-GAPDH antibody [6C5] - Loading Control
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-GAPDH antibody [6C5] - Loading Control
Company
Abcam
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
ab8245
Antibody Type
Primary
Clonality
Monoclonal
Applications
Immunocytochemistry/ Immunofluorescence, Western blot
Host
Mouse, Nude mouse
Quantity
100 UG
Clone
6C5
Isotype
IgG1
Conjugate/Tag
Unconjugated
Format
Purified by chromatography on protein A Sepharose. Purity is tested by electrophoresis
Concentration
2 mg/ml
Gene Name
GAPDH
Reactivity
Human, Mouse, Rat
Target
GAPDH
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (4094)
(1)
GPR37-centered ceRNA network contributes to metastatic potential in lung adenocarcinoma: Evidence from high-throughput sequencing
Translational Oncology
January 1, 2024
Chuanhui Chen, Mengzhi Wan, Xiong Peng, Qing Zhang, Yu Liu
diluted rabbit primary antibodies against GPR37 (MAB44501,
1:1000
, Bio-Techne China, Shanghai) and GAPDH (
ab8245
,
1:2500
,
Abcam
). The next day, the
membrane
was further incubated with HRP-labeled goat anti-mouse IgG
(2)
MK8722 initiates early-stage autophagy while inhibiting late-stage autophagy via FASN-dependent reprogramming of lipid metabolism
Theranostics
January 1, 2024
Luhui Wang, Haiyan Zhu, Zhehao Shi, Bo Chen, Huirong Huang, Ganglian Lin, Jiacheng Li, Haitao Yu, Shihao Xu, Gang Chen,
et al.
Santa Cruz Biotechnology
, 15546-1-AP; proteintech), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (
ab8245
;
Abcam
), CDK1 (19532; Proteintech), Cyclin D1 (26939; Proteintech), Cyclin A2 (18202; Proteintech),
(3)
MALAT1 promotes FOXA1 degradation by competitively binding to miR-216a-5p and enhancing neuroendocrine differentiation in prostate cancer
Translational Oncology
January 1, 2024
Fanchang Zeng, Daoyuan Li, Xinli Kang, Qinghui Wu, Mi Song, Zhewen Ou, Zuobing Yang, Jing Yang, Liumei Luo
Abcam), SYP (
ab32127
,
Abcam
),
FOXA1
(
ab55178
,
Abcam
), ZFP91 (MA5–31,957) and the loading control GAPDH (
ab8245
,
Abcam
), followed by
incubation
with
horseradish
peroxidase-labeled goat anti-mouse IgG (
ab205719
,
1:20,000
).
(4)
Gallic acid improves the metformin effects on diabetic kidney disease in mice
Renal Failure
December 31, 2023
Yan Hong, Jidong Wang, Wenjuan Sun, Lai Zhang, Xuefang Xu, Kaiyue Zhang
EPR19549] to AMPK alpha 1 + AMPK alpha 2,
1:1000
), anti-
SIRT1
(#
ab110304
, 1.25 µg/mL), and anti-GAPDH (#
ab8245
,
1:1000
) from
Abcam
. Protein signals were detected using enhanced chemiluminescence (ECL). The DHE
(5)
MiR-18a-5p attenuates HER2-positive breast cancer development by regulating PI3K/AKT pathway
Cancer Biology & Therapy
December 31, 2023
Yongjun Liu, Hua Yang
(
ab8805
,
Abcam
), P-PI3K (ab278545, Abcam), PI3K (
ab32089
,
Abcam
, UK),
HER2
(
ab134182
,
Abcam
), and GAPDH (
ab8245
,
Abcam
) were incubated with the
membranes
after blocking at 37°C for an hour with 5% nonfat milk. The
(6)
LncRNA TUG1 promotes pulmonary fibrosis progression via up-regulating CDC27 and activating PI3K/Akt/mTOR pathway
Epigenetics
December 31, 2023
Fei Qi, Zhong-Dong Lv, Wen-Di Huang, Shan-Chen Wei, Xin-Min Liu, Wei-Dong Song
(
AP0094
,
1:500
,
ABclonal
), mTOR (
A2445
,
1:500
,
ABclonal
),
CDC27
(
A3333
,
1:1000
,
ABclonal
), and GAPDH (
ab8245
,
1:2000
,
Abcam
) at 4°C overnight, followed by
incubation
with secondary antibody (bs-0295 G-HRP,
1:1000
,
(7)
Pyrotinib and chrysin synergistically potentiate autophagy in HER2-positive breast cancer
Signal Transduct Target Ther
December 18, 2023
Xiaoxiao Liu, Xing Zhang, Zhiying Shao, Xiaorong Zhong, Xin Ding, Liang Wu, Jie Chen, Ping He, Yan Cheng, Kunrui Zhu,
et al.
(ab109012), p-eIEFA (ab157478), XBP-1 (ab37152), IRE (ab37073), G6PD (ab210702), ZBTB16 (ab104854) and GAPDH (
ab8245
), at 4 °C overnight. After washing in Tris-buffered saline with 0.05% Tween 20 (TBST), hybridized
membranes
(8)
Mesenchymal stem cells lose the senescent phenotype under 3D cultivation
Stem Cell Res Ther
December 18, 2023
O. Krasnova, A. Kovaleva, A. Saveleva, K. Kulakova, O. Bystrova, M. Martynova, A. Domnina, J. Sopova, I. Neganova
Goat Anti-Mouse IgG (H + L)-HRP
) (
Abcam
, UK,
ab205719
). As a loading control we used GAPDH (
Abcam
, UK,
ab8245
). All experiments were performed as three biological repeats. Quantifications of
western blot
data were
(9)
Inhibitory effects of the nanoscale lysate derived from xenogenic dental pulp stem cells in lung cancer models
J Nanobiotechnology
December 18, 2023
Yan He, Ruohan Li, Wenting She, Yilong Ai, Kesheng Li, Tushar Kumeria, Ziran Jiang, Qing Shao, Chen Zou, Abdullkhaleg Ali Albashari,
et al.
anti-Bcl-2 (
ab182858
,
1:2000
), anti-Bax (
ab32503
,
1:1000
), anti-Bad (
ab32445
,
1:1000
), anti-GAPDH (
ab8245
,
1:500
) from
Abcam
(Cambridge, MA, USA).
Animal experiments
Male nude
mice
aged 6–8 weeks were obtained
(10)
The 4-1BBζ costimulatory domain in chimeric antigen receptors enhances CD8+ T-cell functionality following T-cell receptor stimulation
Cancer Cell Int
December 18, 2023
Gerard J. Chu, Charles G. Bailey, Rajini Nagarajah, Sharon M. Sagnella, Stephen Adelstein, John E. J. Rasko
appropriate,
Miltenyi
,
130-113-462
CD107a
,FC,REA792,PE,0.3,
Miltenyi
,
130-111-621
Mesothelin,WB,MN1,–,1,Rocklands,200301A88 GAPDH,WB,6C5,–,0.4,
Abcam
,
Ab8245
CD28,Stim,L295,–,1,
BD
,
340975
MSLN,FC,FAB32652P,PE,1,R&D,FAB32652P Rat IgG2a Isotype Control,FC,54447,PE,1,R&D,IC0006P Summary
(11)
Cyclophilin A facilitates influenza B virus replication by stabilizing viral proteins
iScience
December 15, 2023
Huizi Li, Wenhui Fan, Jie Min, Xiaoyuan Bai, Wenxian Yang, Heqiao Li, Yuna Zhao, Runshan Lin, Xiaojuan Jia, Wenjun Liu,
et al.
monoclonal anti-β-tubulin,
Abcam
,Cat#
ab6046
; RRID: AB_2210370 Mouse monoclonal anti-GAPDH,
Abcam
,Cat#
ab8245
; RRID: AB_2107448
Mouse
monoclonal
anti-Tubulin
(
clone
DM1A+DM1B),
Abcam
,Cat#
ab44928
; RRID: AB_2241150
Mouse
(12)
Mitochondrial transplantation reduces lower limb ischemia-reperfusion injury by increasing skeletal muscle energy and adipocyte browning
Molecular Therapy - Methods & Clinical Development
December 14, 2023
Jiaqi Zeng, Jianing Liu, Haiya Ni, Ling Zhang, Jun Wang, Yazhou Li, Wentao Jiang, Ziyu Wu, Min Zhou
anti-OPA1 (Abcepta, AP20727c), UCP1 (Cell Signaling, 722985), β-actin (Abcam,
AB8226
), and GAPDH (
Abcam
,
AB8245
) were incubated overnight at 4°C, followed by
incubation
with appropriate secondary antibodies for 2
(13)
Effective protection of photoreceptors using an inflammation-responsive hydrogel to attenuate outer retinal degeneration
NPJ Regen Med
December 14, 2023
Hyerim Kim, Hyeonhee Roh, Sang-Heon Kim, Kangwon Lee, Maesoon Im, Seung Ja Oh
The
membranes
were incubated with primary antibodies including
1:5000
diluted anti-GAPDH antibody (#
ab8245
,
Abcam
, Cambridege, Cambridgeshire, UK),
1:1000
diluted anti-Phospho-STAT (#
9167
,
Cell Signaling
, Danvers,
(14)
Fasting regulates mitochondrial function through lncRNA PRKCQ-AS1-mediated IGF2BPs in papillary thyroid carcinoma
Cell Death Dis
December 14, 2023
Xiaoping Zhang, Yong Zhong, Lin Liu, Chengyou Jia, Haidong Cai, Jianshe Yang, Bo Wu, Zhongwei Lv
LDHA (
ab52488
,
Abcam
);
HK2
(
ab227198
,
Abcam
);
GLUT1
(
ab115730
,
Abcam
); GPI (
ab210753
,
Abcam
); GAPDH (
ab8245
,
Abcam
); β-actin (
ab8227
,
Abcam
) after blocking with 5% skim milk. Finally, the
membrane
was incubated
(15)
Butyrate reduces adherent-invasive E. coli-evoked disruption of epithelial mitochondrial morphology and barrier function: involvement of free fatty acid receptor 3
Gut Microbes
December 11, 2023
Samira A. Hamed, Armaan Mohan, Saranya Navaneetha Krishnan, Arthur Wang, Marija Drikic, Nicole L. Prince, Ian A. Lewis, Jane Shearer, Åsa V. Keita, Johan D. Söderholm,
et al.
using
mouse
anti-
human
FFAR3 (
1:1,000
; #66811–1-1 g, Proteintech, Rosemount, IL) and GAPDH (
1:1,000
;
ab8245
,
Abcam
) antibodies, and a goat anti-mouse HRP-conjugated secondary antibody (
1:5,000
;
sc-2031
;
Santa Cruz
(16)
Epilepsy-linked kinase CDKL5 phosphorylates voltage-gated calcium channel Cav2.3, altering inactivation kinetics and neuronal excitability
Nat Commun
December 11, 2023
Marisol Sampedro-Castañeda, Lucas L. Baltussen, André T. Lopes, Yichen Qiu, Liina Sirvio, Simeon R. Mihaylov, Suzanne Claxton, Jill C. Richardson, Gabriele Lignani, Sila K. Ultanir
human
neurons
), rabbit anti
CDKL5
1:2000
(
Atlas
HPA002847
, HEK293), mouse anti GAPDH
1:50000
(
Abcam
ab8245
clone
6C5,
mouse
brain &
human
neurons
),
mouse
anti αTubulin
1:100000
(
Sigma
T9026
clone
DM1A,
mouse
(17)
Gardenia Iridoid Glucosides Protect Against α-Naphthalene Isothiocya-Nate-Induced Cholestatic Rats Through Activation of the FXR-SHP Signaling Pathway
Clin Exp Gastroenterol
December 8, 2023
Meng Xu, Ke Che, Cong Wang, Ya-Ru Chen, Meng-Yuan Chen, Guang-Lei Zhang, Hao Yu, Hao-Nan Xu, Ya-Bao Li, Ping Sheng,
et al.
member 4 (
ab272457
), bile salt export pump (
ab155421
) and Glyceraldehyde 3-phosphate dehydrogenase (
ab8245
) protein antibodies were purchased from
Abcam
(USA). Function Enrichment Analysis of
Cholestasis
In
(18)
SOX2-OT Binds with ILF3 to Promote Head and Neck Cancer Progression by Modulating Crosstalk between STAT3 and TGF-β Signaling
Cancers (Basel)
December 8, 2023
Ru Wang, Yifan Yang, Lingwa Wang, Qian Shi, Hongzhi Ma, Shizhi He, Ling Feng, Jugao Fang
ab92355
, dilution rate,
1:1000
), β-actin (
Abcam
,
ab8226
, dilution rate,
1:1000
), and GAPDH (
Abcam
,
ab8245
, dilution rate,
1:1000
) were used
for Western blot
analysis. GAPDH was labeled as an internal reference.
(19)
Exosomal miR-182 derived from bone marrow mesenchymal stem cells drives carfilzomib resistance of multiple myeloma cells by targeting SOX6
J Orthop Surg Res
December 7, 2023
Shifeng Long, Shengping Long, Honglei He, Liang Luo, Mei Liu, Ting Ding
(
ab125011
,
1:1000
,
Abcam
), Calnexin (
ab22595
,
1:1000
,
Abcam
), SOX6 (
ab64946
,
1:1000
, Abcam), and GAPDH (
ab8245
,
1:2000
,
Abcam
). Following primary antibody
incubation
, the
membranes
were once again washed with PBS
(20)
Inhibition of hepatocellular carcinoma growth via modulation of the miR-221/SOX11 axis by curcumin and berberine
PeerJ
December 7, 2023
Sheng Li, Xiaoliang Cai, Liang Chen, Manbian Lin, Ziqi Zhu, Huihuang Xiao, Pingping Nie, Quanwen Chen, Xiaoyu Yang
cleaved
caspase-9
(
1:1,000
, #
9505
;
Cell Signaling Technology
, Danvers, MA, USA), and GAPDH (
1:3,000
,
ab8245
;
Abcam
, Cambridge, UK) primary antibodies overnight at 4 °C.
Membranes
were incubated with goat anti-rabbit
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Reviews
Write a Review
(1)
12/8/2023
Good GAPDH Antibody From Abcam for Western Blotting
Dr. David Sunnen
The specific goal of this western blot experiment was to evaluate the expression level of protein of interest relative to GAPDH protein expression in cell lysate samples. This antibody worked for ...
Read Review
(1)
8/7/2023
Best GAPDH Antibody From Abcam
Shivali Rawat
I use GAPDH antibody as loading control for total cellular protein fractions or soluble protein fractions regularly under various stress conditions.
(1)
9/13/2021
Great GAPDH Antibody
Jian Chen
GAPDH is a common loading control used for most western blots. This antibody has been working great for most of the cell lines we had worked at.
(1)
8/18/2016
Decent Loading Control Antibody
Shikha Saini
Using a loading control is extremely important for any conclusive experiment. Mostly, any gene which is vital for the cell or is abundantly present can be used as a loading control. GAPDH is one of ...
Read Review
(1)
2/1/2016
GAPDH Antibody for Western Blotting
Prudhvi Pusapati
I am interested in studying the role of various transcription factors during regenerative processes of the lung and intestine after chemical induced tissue injury. The level of transcription factors ...
Read Review
(1)
1/11/2016
Western Blotting GAPDH Antibody from Abcam
Ganesh Pusapati
GAPDH is considered like a house keeping gene and so its levels are used as a metric to determine the loading efficiency in gels (unless the experiment affects GAPDH levels. If yes, then an ...
Read Review
(1)
7/15/2015
Review of Anti-GAPDH antibody
Liyong Z.
To perform Western Blot analysis, the cells were washed twice in PBS and harvested with lysis buffer (LB: 20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1 mM Na2EDTA, 1 mM EGTA, 1% Triton X-100, 2.5 mM sodium ...
Read Review
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Anti-GAPDH antibody [6C5] - Loading Control from Abcam
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
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