Great Antibody to Detect GAPDH

May 08, 2020

Pharmacology
Duke University
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Cell Signaling Technology

Product Name:

GAPDH

Catalog Number:

5174

Clone Number:

D16H11

This antibody worked to detect GAPDH and provided one band at the correct molecular weight.

Experimental Design and Results Summary

Applications

Western Blot

Sample

MDA-MB-231 Cells

Primary Incubation

1:2000

Blocking Agent

5%NDS

Secondary Incubation

1:1000

Tertiary Incubation

None

Detection

Chemiluminescence

Results Summary

This worked well and had minimal to no background and can be used at very low dilutions.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

This works well

The Bad

None

The Bottom Line

This is great to detect GAPDH.

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