GAPDH Antibody AF5718 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemGAPDH Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $529.00Supplier Page View Company Product Page
Catalog NumberAF5718
NCBI Gene AliasesGAPD, HEL-S-162eP, G3PD
HostGoat
IsotypeIgG
ApplicationsWestern Blot Control,Flow Cytometry,Immunohistochemistry,Western Blot,Simple Western,Immunocytochemistry
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
ReactivityHamster,Human,Mouse,Rat,Transgenic Mouse
TargetGAPDH
NCBI Full Gene Nameglyceraldehyde-3-phosphate dehydrogenase
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant human GAPDH/G3PDH, Met1-Ala150, Accession # P04406
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human, mouse, and rat GAPDH/G3PDH in direct ELISAs. In direct ELISAs, approximately 25% cross-reactivity with recombinant human GAPDH-2 is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this GAPDH antibody be used for Western blotting in my experiments?

Yes, the GAPDH antibody has been shown to work effectively in Western blotting applications, as evidenced by its use in multiple studies, including those that quantified protein bands normalized to GAPDH levels (see , , ).

Is this antibody suitable for use in immunocytochemistry?

The GAPDH antibody is applicable for immunocytochemistry, as indicated by its inclusion in various research studies that utilized this technique to visualize GAPDH expression in different cell types (see , ).

What species can this antibody detect?

This GAPDH antibody is reactive with human, mouse, rat, and hamster GAPDH, making it versatile for studies involving these species (see product specifications).

How does this antibody perform in terms of specificity?

The GAPDH antibody has been reported to detect human, mouse, and rat GAPDH with approximately 10% cross-reactivity with recombinant human GAPDH-2 in direct ELISAs, indicating a high level of specificity for its intended targets (see product specifications).

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) ESRP2 constrains EMT plasticity associated with ZEB1 expression in bladder cancerFrontiers in OncologyMarch 30, 2026Karolina Bajdak-Rusinek, Karolina Jankowska, Vignesh Sundararajan, Łukasz Sieroń, Natalia Diak, Weronika Wójtowicz, Karolina L. Stępień, Agnieszka Fus-Kujawa, Ewa Gutmajster, Mateusz Wierzbinka, et al.(Invitrogen, OMA1-06001; 1:3000), ZEB1 (Bethyl, A301921A; 1:1000) and loading control GAPDH (R&D Systems, AF5718; 1:5000), followed by HRP-conjugated secondary antibodies (Jackson ImmunoResearch, West Grove, PA; 1:25,000).

(2) A Modular Bioinstructive Platform Reveals Mechanistic Insights into Additive‐Free, Topography‐Driven OsteogenesisAdvanced Healthcare MaterialsMarch 1, 2026Fatmah I. Ghuloum, Leo A. H. Zeef, Lee A. Stevens, Marco A. N. Domingos, Susan J. Kimber, Mahetab H. AmerAB_2233454) and goat anti‐Glyceraldehyde‐3‐Phosphate Dehydrogenase (GAPDH) polyclonal antibody (1:1000, AF5718, R&D Systems, RRID: AB_2278695) were used simultaneously for primary detection. Secondary detection

(3) Factor-H-related protein 1 (FHR1), a promotor of para-inflammation in age-related macular degenerationJournal of neuroinflammationJuly 3, 2025Sekulic A, Herr SM, Mulfaul K, Pompös IM, Winkler S, Dietrich C, Obermayer B, Mullins RF, Conrad T, Zipfel PF, et al.dilutions indicated: anti-muFHR1 (generated by DAVIDS Biotechnologies), and anti-GAPDH (R&D Systems, #AF5718) at 1:1000, anti-FHR1 (generated by DAVIDS Biotechnologies), anti-CFH (sc-166608-HRP, Santa Cruz), anti-EMR2

(4) Fatty acid synthase global inducible knockout does not alter brain fatty acid concentrations but attenuates cholesterol synthesis in the adult mouseProstaglandins, Leukotrienes and Essential Fatty AcidsJuly 1, 2025Drew R. Seeger, Peddanna Kotha, Svetlana A. Golovko, Eric J. Murphy, Mikhail Y. GolovkoRockford, IL, cat.no. PA5–109705), goat polyclonal GAPDH 1:1000 (R&D Systems, Minneapolis, MN, cat.no. AF5718), mouse monoclonal β-Actin 1:1000 ((R&D Systems, Minneapolis, MN, cat.no. MAB8929), goat polyclonal

(5) Homeostatic regulation of nucleoporins is a central driver of nuclear pore biogenesisCell ReportsApril 22, 2025Stephen Sakuma, Marcela Raices, Ethan Y.S. Zhu, Dana Mamriev, Charles I. Fisher, Susanne Heynen-Genel, Maximiliano A. D’AngeloAB_331355 TOM70 Mouse mAb,Cell Signaling,Cat#: 98445; RRID: AB_3674095 GAPDH Goat pAb,R&D Systems,Cat#: AF5718; RRID: AB_355053 Alexa Fluor 488 Anti-Mouse IgG Polyclonal,Invitrogen,Cat#: A21202; RRID: AB_141607 Alexa

(6) Cryo-TEM structure of β-glucocerebrosidase in complex with its transporter LIMP-2Nat CommunMarch 30, 2025Jan Philipp Dobert, Jan-Hannes Schäfer, Thomas Dal Maso, Priyadarshini Ravindran, Dustin J. E. Huard, Eileen Socher, Lisa A. Schildmeyer, Raquel L. Lieberman, Wim Versées, Arne Moeller, et al.#66003-1-lg; dilution: 1:1,000), goat anti GAPDH (polyclonal, R&D Systems, Minneapolis, MN, United States, #AF5718); Secondary: IRDye 800CW donkey anti goat (LI-COR Biosciences, Lincoln, NE, United States, #926-32214;

(7) TBCK-deficiency leads to compartment-specific mRNA and lysosomal trafficking defects in patient-derived neuronsbioRxivMarch 7, 2025Marco Flores-Mendez, Jesus A. Tintos-Hernández, Leonardo Ramos-Rodriguez, Leann Miles, Tsz Y Lo, Yuanquan Song, Xilma R. Ortiz-GonzálezCell signaling, 72610), mouse anti-Flag (1:1000, Sigma, F1804), goat anti-GAPDH (1:2000, RD Systems, AF5718), rabbit anti-TBCK (1:1000, Novus, NBP1–83166), rabbit anti-VDAC (1:1000, Abcam, ab15895), mouse anti-LAMP1

(8) Endothelial cell Piezo1 promotes vascular smooth muscle cell differentiation on large arteriesEuropean Journal of Cell BiologyMarch 1, 2025Javier Abello, Ying Yin, Yonghui Zhao, Josh Maurer, Jihui Lee, Cherokee Bodell, Jahmiera Richee, Abigail J. Clevenger, Zarek Burton, Megan E. Goeckel, et al.(ThermoScientific, Cat. #: 21059) for 15 minutes at room temperature. GAPDH anti-goat IgG (Abcam, Cat. #: AF5718) 1:5000 dilution, followed by secondary anti-goat HRP (R&D systems, Cat.#: HAF019) 1:5000 dilution was

(9) AZD6738 Attenuates LPS-Induced Corneal Inflammation and Fibrosis by Modulating Macrophage Function and PolarizationInflammationFebruary 4, 2025Longxiang Huang, Youfang Luo(2117, rabbit monoclonal antibody, 1:1000; Cell Signaling Technology, Danvers, MA, USA), and GAPDH (AF5718, goat polyclonal antibody, 1 mg/mL; R&D Systems, Minneapolis, MN, USA) overnight at 4 ℃. After washing

(10) The role of atropine in myopia control: insights into choroidal and scleral mechanismsFrontiers in pharmacologyJanuary 1, 2025Huang L, Zhang J, Luo Y.rabbit monoclonal antibody, 1:1000; Cell Signaling Technology, Danvers, MA, United States), and GAPDH (AF5718, goat polyclonal antibody, 1 mg/mL; R&D Systems, Minneapolis, MN, United States) overnight at 4°C. After

(11) PARP trapping is governed by the PARP inhibitor dissociation rate constantCell Chemical BiologyJuly 18, 2024Angelica A. Gopal, Bianca Fernandez, Justin Delano, Ralph Weissleder, J. Matthew Dubach,Cell Signaling Technology,Cat#2577 Histone H3 antibody,Cell Signaling Technology,Cat#9715 GADPH antibody,R&D Systems,Cat#AF5718 RPA1 antibody,Cell Signaling Technology,Cat#2267 BRCA1 antibody,Thermo Fisher,Cat#MA1-23164 Dnk pAb

(12) Late‐onset Krabbe disease presenting as spastic paraplegia – implications of GCase and CTSB/DAnnals of Clinical and Translational NeurologyJuly 1, 2024Rebecca Mächtel, Jan‐Philipp Dobert, Ute Hehr, Alexander Weiss, Matthias Kettwig, Lucia Laugwitz, Samuel Groeschel, Manuel Schmidt, Philipp Arnold, Martin Regensburger, et al.(#610800)Anti‐beta‐Actin antibody (mouse), abcam (#ab5441)Human/Mouse/Rat GAPDH Antibody, R&D Systems (#AF5718)Donkey anti‐Mouse IgG (H + L) Highly Cross‐Adsorbed Secondary Antibody, Alexa Fluor™ 680, ThermoFisher Scientific

(13) Dysfunction of Small-Conductance Ca2+-Activated Potassium (SK) Channels Drives Amygdala Hyperexcitability and Neuropathic Pain Behaviors: Involvement of Epigenetic MechanismsCellsJune 18, 2024Vadim Yakhnitsa, Jeremy Thompson, Olga Ponomareva, Guangchen Ji, Takaki Kiritoshi, Lenin Mahimainathan, Deborah Molehin, Kevin Pruitt, Volker Neugebauerprimary antibodies (α-SK2 subunit, Guinea Pig, Alomone AGP-045, 1:1000 and α-GAPDH, Goat, R&D systems, AF5718, 1:2000) at 4 °C overnight and then in secondary polyclonal antibodies (Donkey α–Rabbit, IRDye 680LT,

(14) Distinct CD16a features on human NK cells observed by flow cytometry correlate with increased ADCCScientific reportsApril 4, 2024Benavente MCR, Hakeem ZA, Davis AR, Murray NB, Azadi P, Mace EM, Barb AW.

(15) Regulation of angiogenesis by endocytic trafficking mediated by cytoplasmic dynein 1 light intermediate chain 1bioRxiv : the preprint server for biologyApril 1, 2024Johnson D, Colijn S, Richee J, Yano J, Burns M, Davis AE, Pham VN, Saric A, Jain A, Yin Y, et al.

(16) Cathepsin V regulates cell cycle progression and histone stability in the nucleus of breast cancer cellsFrontiers in PharmacologyNovember 6, 2023Naphannop Sereesongsaeng, James F. Burrows, Christopher J. Scott, Klaudia Brix, Roberta E. BurdenNASP (ab181169, Abcam), mouse monoclonal GATA3 (BioTechne, MAB6330), goat polyclonal GAPDH (BioTechne, AF5718), mouse monoclonal CTSL (BioTechne, MAB9521) and rat monoclonal α-Tubulin (ab6160, Abcam). Secondary

(17) Enhancing viability and angiogenic efficacy of mesenchymal stem cells via HSP90α and HSP27 regulation based on ROS stimulation for wound healingBioengineering & translational medicineSeptember 1, 2023Seo I, Kim SW, Hyun J, Kim YJ, Park HS, Yoon JK, Bhang SH.to immuno‐blot polyvinylidene fluoride membranes (Bio‐Rad) and probed with antibodies against GAPDH (AF5718, 1:2000; R&D Systems, Minneapolis, MN, USA), CASPASE‐3 (#9662, 1:1000; Cell Signaling Technology, MA,

(18) Discovery and Characterization of ZL-2201, a Potent, Highly Selective, and Orally Bioavailable Small-molecule DNA-PK InhibitorCancer Res CommunSeptember 1, 2023Lal S, Bhola NE, Sun BC, Chen Y, Huang T, Morton V, Chen KX, Xia S, Zhang H, Parikh NS, et al.CST2577), H2AX (RRID:AB_2118009; Cell Signaling Technology; CST9718), GAPDH (RRID:AB_2278695; R&D Systems; AF5718), pMCM2 (S108; RRID:AB_242513; Bethyl Lab; A300-094A), vinculin (RRID:AB_10559207; Cell Signaling Technology;

(19) Different activity patterns control various stages of Reelin synthesis in the developing neocortexCerebral cortex (New York, N.Y. : 1991)July 24, 2023Engeroff K, Warm D, Bittner S, Blanquie O.incubated overnight at 4°C in mouse anti-Reelin G10 (MAB5364, Millipore 1:1,000) or goat anti-GAPDH (AF5718, R&D System, 1:5,000). After 3 10-min washes in TBS-T, membranes were incubated with an horseradish

(20) Glomerulonephritis and autoimmune vasculitis are independent of P2RX7 but may depend on alternative inflammasome pathwaysThe Journal of pathologyJuly 1, 2022Prendecki M, McAdoo SP, Turner-Stokes T, Garcia-Diaz A, Orriss I, Woollard KJ, Behmoaras J, Cook HT, Unwin R, Pusey CD, et al.transferred to nitrocellulose membranes, and probed with the following primary antibodies: GAPDH (1:1,000, AF5718, R&D Systems, Minneapolis, MN, USA), P2RX7 004 (1:200, APR004, Alomone, Jerusalem, Israel), IL‐1β (1:1,000,

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GAPDH Antibody

GAPDH Antibody

GAPDH Antibody

GAPDH Antibody

GAPDH Antibody

GAPDH Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

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GAPDH Antibody from R&D Systems, a Bio-Techne Brand

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