Anti-GAPDH antibody [6C5] - Loading Control ab8245 from Abcam

Product Specs
ItemAnti-GAPDH antibody [6C5] - Loading Control
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab8245
Antibody TypePrimary
ClonalityMonoclonal
ApplicationsImmunocytochemistry/ Immunofluorescence, Western blot
HostMouse, Nude mouse
Quantity100 UG
Clone6C5
IsotypeIgG1
Conjugate/TagUnconjugated
FormatPurified by chromatography on protein A Sepharose. Purity is tested by electrophoresis
Concentration2 mg/ml
Gene NameGAPDH
ReactivityHuman, Mouse, Rat
TargetGAPDH

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(1) LncRNA TUG1 promotes pulmonary fibrosis progression via up-regulating CDC27 and activating PI3K/Akt/mTOR pathwayEpigeneticsDecember 31, 2023Fei Qi, Zhong-Dong Lv, Wen-Di Huang, Shan-Chen Wei, Xin-Min Liu, Wei-Dong Song(AP0094, 1:500, ABclonal), mTOR (A2445, 1:500, ABclonal), CDC27 (A3333, 1:1000, ABclonal), and GAPDH (ab8245, 1:2000, Abcam) at 4°C overnight, followed by incubation with secondary antibody (bs-0295 G-HRP, 1:1000,

(2) MiR-18a-5p attenuates HER2-positive breast cancer development by regulating PI3K/AKT pathwayCancer Biology & TherapyDecember 31, 2023Yongjun Liu, Hua Yang(ab8805, Abcam), P-PI3K (ab278545, Abcam), PI3K (ab32089, Abcam, UK), HER2 (ab134182, Abcam), and GAPDH (ab8245, Abcam) were incubated with the membranes after blocking at 37°C for an hour with 5% nonfat milk. The

(3) Gallic acid improves the metformin effects on diabetic kidney disease in miceRenal FailureDecember 31, 2023Yan Hong, Jidong Wang, Wenjuan Sun, Lai Zhang, Xuefang Xu, Kaiyue ZhangEPR19549] to AMPK alpha 1 + AMPK alpha 2, 1:1000), anti-SIRT1 (#ab110304, 1.25 µg/mL), and anti-GAPDH (#ab8245, 1:1000) from Abcam. Protein signals were detected using enhanced chemiluminescence (ECL). The DHE

(4) HYAL1 deficiency attenuates lipopolysaccharide-triggered renal injury and endothelial glycocalyx breakdown in septic AKI in miceRenal failureDecember 1, 2023Xing H, Li S, Fu Y, Wan X, Zhou A, Cao F, Sun Q, Hu N, Ma M, Li W, et al.anti-LC3-I (ab62721; 1:1000), anti-LC3-II (ab192890; 1:2000), anti-p62 (ab109012; 1:10000) and GAPDH (ab8245; 1:500) at 4 °C overnight and subsequently with horseradish peroxidase-labeled IgG (ab6721; 1:1000)

(5) DHX9 Strengthens Atherosclerosis Progression By Promoting Inflammation in MacrophagesInflammationOctober 1, 2023Huangfu N, Ma H, Tian M, Zhang J, Wang Y, Li Z, Chen X, Cui H.Proteintech), p65 (No.66535–1-Ig, Proteintech), p65 (phospho S536) (p-p65, sc-136548, Santa Cruz), GAPDH (ab8245, Abcam), flag (ab205606, Proteintech), or Lamin B1 (No.66095–1-Ig, Proteintech). After incubating with

(6) Kappa opioid receptor in nucleus accumbens regulates depressive-like behaviors following prolonged morphine withdrawal in miceiScienceSeptember 15, 2023Jinyu Zhang, Ye Lu, Min Jia, Yuying Bai, Lulu Sun, Ziqing Dong, Wenrong Tian, Fangyuan Yin, Shuguang Wei, Yunpeng Wanganti-Synaptophysin,Servicebio,Cat# GB11553; RRID: AB_2937063 Mouse monoclonal anti-GAPDH,Abcam,Cat# ab8245; RRID:AB_2107448 Cy3 Goat Anti-Mouse IgG,Abclonal,Cat#AS008; RRID:AB_2769088 Fluorescein Anti-Avidin

(7) circAGTPBP1 promotes the progression of papillary thyroid cancer through the notch pathway via the miR-34a-5p/notch1 axisiScienceSeptember 15, 2023Dai L, Zhang W, Wang Y, Yu K, Le Q, Wu X.Abcam), anti-Hes1 (cat. #ab71559, Abcam), anti-Jagged1 (cat. #ab300561, Abcam), and anti-GAPDH (cat. #ab8245, Abcam) overnight at 4°C. Then, the membranes were incubated with secondary antibody (Abcam) for 2 h.

(8) Human genetic adaptation related to cellular zinc homeostasisPLoS geneticsSeptember 1, 2023Roca-Umbert A, Garcia-Calleja J, Vogel-González M, Fierro-Villegas A, Ill-Raga G, Herrera-Fernández V, Bosnjak A, Muntané G, Gutiérrez E, Campelo F, et al.diluted in the corresponding blocking solution anti-HA for ZnT9 (H3663, Sigma-Merck), and anti-GAPDH (ab8245, Abcam). HRP secondary antibodies (1:1000; GE Healthcare) were used depending on the primary antibody.

(9) S100A5 Attenuates Efficiency of Anti-PD-L1/PD-1 Immunotherapy by Inhibiting CD8(+) T Cell-Mediated Anti-Cancer Immunity in Bladder CarcinomaAdvanced science (Weinheim, Baden-Wurttemberg, Germany)September 1, 2023Li H, Chen J, Li Z, Chen M, Ou Z, Mo M, Wang R, Tong S, Liu P, Cai Z, et al.overnight with primary antibodies: anti‐S100A5 (Cat: 17924‐1‐AP, Proteintech, USA) and anti‐GAPDH (Cat: ab8245, Abcam, USA). After washing with TBST for three times, the PVDF membrane was incubated at room temperature

(10) Site-specific phosphorylations of the Arf activator GBF1 differentially regulate GBF1 function in Golgi homeostasis and secretion versus cytokinesisSci RepAugust 21, 2023Kendall Walton, Tomasz J. Nawara, Allyson R. Angermeier, Hadley Rosengrant, Eunjoo Lee, Bridge Wynn, Ekaterina Victorova, George Belov, Elizabeth Sztulsecondary antibodies were used at a 1:5000 concentration. Antibodies Mouse monoclonal anti-GAPDH (ab8245) and rabbit polyclonal anti-GFP (ab290) were purchased from Abcam (Cambridge, MA). Mouse monoclonal

(11) MIF is a critical regulator of mononuclear phagocytic infiltration in hepatocellular carcinomaiScienceAugust 18, 2023Yunxi Liao, Chenyang Wu, Yang Li, Jinhua Wen, Dongyu ZhaoRESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Anti-SPP1,Abcam,Cat# ab214050 Anti-MIF,Abcam,Cat# ab187064 Anti-GADPH,Abcam,Cat# ab8245 HRP-labeled Goat Anti-Rabbit IgG (H+L),Beyotime,Cat# A0208 ,, Bacterial and virus strains,Bacterial

(12) Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1iScienceAugust 18, 2023Tong L, Rao J, Yang C, Xu J, Lu Y, Zhang Y, Cang X, Xie S, Mao J, Jiang P.RRID:AB_2915918 NGAL,Proteintech,Cat#26991-1-AP; RRID:AB_2880715 XPNPEP3,Genetex,Cat#GTX105541; RRID:AB_1952597 GAPDH,Abcam,Cat# ab8245; RRID:AB_2107448 TOM20,Abclonal,Cat#A19403; RRID:AB_2862646 UPF1,Proteintech,Cat#23379-1-AP; RRID:AB_11232421 DCP2,Abclonal,Cat#A8282;

(13) Unraveling the Role of EV-Derived miR-150-5p in Prostate Cancer Metastasis and Its Association with High-Grade Gleason Scores: Implications for DiagnosisCancers (Basel)August 17, 2023Marian Cruz-Burgos, Sergio A. Cortés-Ramírez, Alberto Losada-García, Miguel Morales-Pacheco, Eduardo Martínez-Martínez, Jorge Gustavo Morales-Montor, Alejandro Servín-Haddad, J. Samuel Izquierdo-Luna, Griselda Rodríguez-Martínez, María Del Pilar Ramos-Godínez, et al.(Thermo Fisher Scientific, Inc., cat. 10628D), anti-TSG101 (Abcam, Cambridge, UK, cat. cab83), anti-GAPDH (Abcam, cat. ab8245), anti-β-actin (Abcam, cat. ab8221), anti-rabbit HRP secondary (Abcam, cat. ab97051), and anti-mouse

(14) Rac GTPase activating protein 1 promotes the glioma growth by regulating the expression of MCM3Transl OncolAugust 16, 2023Bo Jia, Yuran Jiang, Yu Huan, Yu Han, Wei Liu, Xiao Liu, Yingwen Wang, Lei He, Zhengcong Cao, Xin He, et al.13739-1-AP and Abcam, cat. no. ab97315), GAPDH (Proteintech, cat no. 60004-1-Ig and Abcam, cat. no. ab8245); MCM3 (Proteintech, cat no. 15597-1-AP); YY1 (Proteintech, cat no. 66,281–1-Ig), goat anti-mouse IgG

(15) PARP1-catalyzed PARylation of YY1 mediates endoplasmic reticulum stress in granulosa cells to determine primordial follicle activationCell Death DisAugust 15, 2023Wei Chen, Qiukai E, Bo Sun, Pengxue Zhang, Nan Li, Shujia Fei, Yingnan Wang, Shuting Liu, Xiaoqiu Liu, Xuesen Zhanganti-HA-tag (1:1000, #30701ES60, YEASEN), anti-FLAG-tag (1:1000, #30501ES60, YEASEN) and anti-GAPDH (1:4000, #ab8245, Abcam, Cambridge, UK) antibodies overnight at 4 °C. After TBST washing, the membranes were treated

(16) Programming conformational cooperativity to regulate allosteric protein-oligonucleotide signal transductionNat CommunAugust 14, 2023Yuan Liang, Yunkai Qie, Jing Yang, Ranfeng Wu, Shuang Cui, Yuliang Zhao, Greg J. Anderson, Guangjun Nie, Suping Li, Cheng Zhangpenicillin/streptomycin (Sigma-Aldrich). Primary antibodies against PLK1 (EPR19534(ab189139)) and GAPDH (6C5(ab8245)) were purchased from Abcam (Cambridge, UK). HRP-linked goat anti-rabbit secondary antibodies (PR30011)

(17) A Comparative Study on the Lysosomal Cation Channel TMEM175 Using Automated Whole-Cell Patch-Clamp, Lysosomal Patch-Clamp, and Solid Supported Membrane-Based Electrophysiology: Functional Characterization and High-Throughput Screening Assay DevelopmentInt J Mol SciAugust 14, 2023Andre Bazzone, Maria Barthmes, Cecilia George, Nina Brinkwirth, Rocco Zerlotti, Valentin Prinz, Kim Cole, Søren Friis, Alexander Dickson, Simon Rice, et al.for 24 h: rabbit anti-TMEM175 (Proteintech 19925-1-AP, dilution 1:1000) and mouse anti-GAPDH (Abcam ab8245, dilution: 1:5000). Subsequently, the secondary polyclonal antibody Goat anti-rabbit (Li-Cor 926-32211,

(18) ZNF143 inhibits hepatocyte mitophagy and promotes non-alcoholic fatty liver disease by targeting increased lncRNA NEAT1 expression to activate ROCK2 pathwayEpigeneticsAugust 11, 2023Yujie Dong, Minjie Hu, Kewei Tan, Rongjuan Daifollowed by detecting the signals with enhanced chemiluminescence reagent (EMD Millipore). GAPDH (1:10000, ab8245, Abcam) used as a control. Statistical analysis Data in this study was presented as mean ± standard

(19) A mannitol-modified emodin nano-drug restores the intestinal barrier function and alleviates inflammation in a mouse model of DSS-induced ulcerative colitisChin MedAugust 11, 2023Yin-Yue Xu, Min Zhu, Jiang Wu, Long-Biao Luo, Si-Jing Dong, Meng-Gai Zhang, Xue Liu, Ke Wang, Hua Luo, Wang-Hui Jing, et al.ZO-1 (1:1000, Cat #ab276131; Abcam), Occludin (1:2000, Cat #ab216327; Abcam), and GAPDH (1:5000, Cat #ab8245; Abcam) at 4 °C. The following day, the membranes were incubated with secondary antibodies and developed

(20) N6-methyladenosine (m6A) methyltransferase WTAP-mediated miR-92b-5p accelerates osteoarthritis progressionCell Commun SignalAugust 10, 2023Zhaowei Lin, Tao Jiang, Wei Zheng, Jiayuan Zhang, Anan Li, Chao Lu, Wengang Liu1:1000, Abcam), anti-Aggrecan (ab3778, 1:750, Abcam), anti-TIMP4 (ab58425, 1:750, Abcam), and anti-GADPH (ab8245, 1:1000, Abcam). Total RNA m6A quantification Total RNA was separated using TRIzol reagent (Takara,

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12/8/2023Good GAPDH Antibody From Abcam for Western BlottingDr. David SunnenThe specific goal of this western blot experiment was to evaluate the expression level of protein of interest relative to GAPDH protein expression in cell lysate samples. This antibody worked for ... Read Review
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8/7/2023Best GAPDH Antibody From Abcam Shivali RawatI use GAPDH antibody as loading control for total cellular protein fractions or soluble protein fractions regularly under various stress conditions.
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9/13/2021Great GAPDH Antibody Jian ChenGAPDH is a common loading control used for most western blots. This antibody has been working great for most of the cell lines we had worked at.
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8/18/2016Decent Loading Control Antibody Shikha SainiUsing a loading control is extremely important for any conclusive experiment. Mostly, any gene which is vital for the cell or is abundantly present can be used as a loading control. GAPDH is one of ... Read Review
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2/1/2016GAPDH Antibody for Western Blotting Prudhvi PusapatiI am interested in studying the role of various transcription factors during regenerative processes of the lung and intestine after chemical induced tissue injury. The level of transcription factors ... Read Review
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1/11/2016Western Blotting GAPDH Antibody from Abcam Ganesh PusapatiGAPDH is considered like a house keeping gene and so its levels are used as a metric to determine the loading efficiency in gels (unless the experiment affects GAPDH levels. If yes, then an ... Read Review
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7/15/2015Review of Anti-GAPDH antibody Liyong Z.To perform Western Blot analysis, the cells were washed twice in PBS and harvested with lysis buffer (LB: 20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1 mM Na2EDTA, 1 mM EGTA, 1% Triton X-100, 2.5 mM sodium ... Read Review

Anti-GAPDH antibody [6C5] - Loading Control from Abcam

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Anti-GAPDH antibody [6C5] - Loading Control from Abcam

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