Good Buffer to Lyse Cells for Protein Detection

June 12, 2023

BlueskyReddit
Dana-Farber Cancer Institute
CIV
Postdoctoral Researcher

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Company:

Thermo Fisher Scientific

Product Name:

RIPA Lysis and Extraction Buffer

Catalog Number:

89900

It is ready-to-use solution for getting extraction of cells, does not need to assemble, and is very fast.

Experimental Design and Results Summary

Application

It is a cell lysis reagent for cultured mammalian cells.

Starting Material

N/A

Tips

Let the incubation lasts for 10 to 20 minutes

Results Summary

Wash the cells, incubate them with the buffer for 10 to 20 minutes, spin down, and take the cell extraction from supernatant, very easy!

DOI or PMID #

N/A

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Summary

The Good

Very easy to use.

The Bad

Nothing.

The Bottom Line

According to the products website "It is effectively lyses and extracts protein from cultured mammalian cells, including plated cells and pelleted suspension cells. The popular reagent enables the extraction of membrane, nuclear and cytoplasmic proteins". Taking the extraction from nuclear is one big advantages for so many of studies.

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