Buffer Set Helps Make Cells Permeable for Intracellular Staining

Dana-Farber Cancer Institute
CIV
Postdoctoral Researcher

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Company:

BioLegend

Product Name:

Cyto-Fast™ Fix/Perm Buffer Set

Catalog Number:

426803

This product has both Cyto-Fast™ Fix Perm Solution and Cyto-Fast™ Perm Wash Solution (10X). It can be used for fixation and permeabilization of mammalian cells for intracellular staining such as cytokines and other cytoplasmic molecules.

Experimental Design and Results Summary

Application

Fixation and permeabilization of mammalian cells

Starting Material

N/A

Tips

Keep the cells in the dark.

Results Summary

Mammalian cells can be station (surface markers), fixed, then permeabilized with this product. After that, intracellular markers can be stained

DOI or PMID #

None

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Summary

The Good

Very little damage to the cells.

The Bad

Nothing.

The Bottom Line

It makes it possible to stain the intercellular markers in the cells that have already been stained for their surface markers.

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