Good SLC22A2 Antibody for Organic Cation Transporter 2

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Rabbit Anti-SLC22A2

Catalog Number:

ab170871

Clone Number:

N/A

Info:Supplier Page View Product Specs

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My research involves studying how D1 dopamine receptor, a member of the GPCR superfamily, can signal from distinct membrane compartments within the cell. I have found that D1 dopamine receptor can be activated at the plasma membrane and from endosomes after internalization from the cell surface. I have also found that this receptor exists as a stable pool at the Golgi apparatus and can signal from there as well.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HeLa and HEK293 cell lysates

Primary Incubation

1:1000, overnight, 4degree C on shaker

Blocking Agent

3% milk in TBS-Tween

Secondary Incubation

1:10000, 1hr, RT on shaker

Tertiary Incubation

chemiluminescent substrates, 2min RT

Detection

ECL then film/image dock

Results Summary

This product helped us find which cell types (between HeLa and HEK293) express Organic Cation Transporter 2.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Clean, works well.

The Bad

Nothing

The Bottom Line

Great signal, recommend this product.

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