Excellent CD20 Antibody for Immunohistochemistry

Afyon Kocatepe University

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

CD20 Rabbit Polyclonal Antibody, Epredia™

Catalog Number:

Thermo RB-9013-P

Clone Number:

Polyclonal, C-terminus of human CD20 protein

Info:Supplier Page 

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This antibody is excellent for mammalian species on formol fixed paraffin embedded tissue Immunohistochemical stains.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Canine lymph node

Primary Incubation

1/300, RT, 2 hours

Blocking Agent

BSA, 1%

Secondary Incubation

Biotinylated Rabbit IgG, RT, 2 hours

Tertiary Incubation

Streptavidin-peroxidase, RT, 1 hours / AEC chromagen, 20', RT

Detection

ABC-peroxidase method

Results Summary

I usually use it for phenotyping of canine and feline lymphomas. In this sense, it is a very successful antibody. Legends: great membranous positivity on B lymphocytes.

DOI or PMID #

N/A

Additional Notes

Gill's hematoxylin as a background staining

Image Gallery

Summary

The Good

Specific, sharp and working multiple species.

The Bad

Nope

The Bottom Line

This antibody is excellent for mammalian species on formol fixed paraffin embedded tissue Immunohistochemical stains.

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