Antibody Stains Ly6G Very Well

Stanford

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 570™ anti-mouse Ly-6G Antibody

Catalog Number:

127629

Clone Number:

1A8

Info:Supplier Page View Product Specs

Browse Similar Products: Ly6g Antibodies

We wanted to assess how certain perturbations would affect neutrophils in the spleen, so we used the antibody to identify Ly6G+ immune cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mouse spleen

Primary Incubation

1:100

Blocking Agent

Fc Block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Cytek Aurora

Results Summary

We stained mouse spleen cells with the anti-Ly6G antibody and found that it identifies Ly6G+ neutrophils very well. The antibody was easy to use and the staining was very clear.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

The antibody works great for staining Ly6G.

The Bad

None to mention.

The Bottom Line

The antibody allowed easy identification of neutrophils in the spleen.

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