Excellent TUBB3 Antibody for Immunofluorescence

June 14, 2023

Wayne State University

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Purified anti-Tubulin beta 3 (TUBB3) from BioLegend

Catalog Number:

801201

Clone Number:

TUJ1

Very good for immunofluorescence staining of mouse enteric neurons. High specificity with low background.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse intestine muscularis

Primary Incubation

1:500 overnight at 4C

Blocking Agent

PBST + 5% donkey serum

Secondary Incubation

1:500, 1 hour at room temperature

Tertiary Incubation

N/A

Detection

ZEISS LSM880

Results Summary

Fluorescence signal from myenteric plexus is very strong. There is little background, allowing excellent visualization of the enteric neurons.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Great staining.

The Bad

N/A

The Bottom Line

This antibody works wonderfully for intrinsic enteric neurons.

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