Anti-Vimentin antibodies are essential reagents in flow cytometry for detecting the protein encoded by the VIM gene. Vimentin is an intermediate filament protein that contributes to the formation and structure of the cytoskeleton. It supports and anchors organelles in the cytosol, attaching to the nucleus, endoplasmic reticulum, and mitochondria. Vimentin has been used as a phenotypic marker for mesenchymal cells, astrocytes, neural stem cells, and senescent cells. Examine the supplier's provided validation data when comparing Vimentin antibodies for flow cytometry. Also look for application-specific recommendations, such as dilutions, protocols, sample types, and other helpful usage notes.