This Anti-Vimentin Antibody From BioLegend Possess Specific Staining

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Company:

BioLegend

Product Name:

Anti-Vimentin Antibody

Catalog Number:

677802

Clone Number:

091D3

Specific staining of astrocytes and vasculature in fresh-frozen human brain tissue. Used at 1:200 dilution with goat anti-mouse AF647 secondary antibody.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Fresh frozen human brain

Primary Incubation

overnight at 4C

Blocking Agent

TNB buffer

Secondary Incubation

2 hours at RT

Tertiary Incubation

None

Detection

Fluorescent microscopy

Results Summary

Immunofluorescent images show specific staining of astrocytes and vasculature in the human fresh frozen brain tissue. Anti-Vimentin antibody was diluted 1:200 and used with goat anti mouse AF647 secondary antibody (diluted 1:800).

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Specific staining, minimal background noise, and not too expensive.

The Bad

None.

The Bottom Line

Would definitely recommend.

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