Fig 1: VIM-depleted weakened HeLa cells’ migration and invasion abilities. (A) CCAT1 downregulation did not influence the mRNA expression level of VIM gene. (B) Vimentin protein level was decreased accompanied by CCAT1 down-regulation. (C)VIM gene was downregulated using RNA interfering technique. The mRNA expression level of VIM gene was downregulated successfully. (D) Vimentin protein level was downregulated successfully using RNA interfering technique (right). (E) VIM knocked down significantly weakened HeLa cells’ migration ability. (F) VIM knocked down significantly weakened HeLa cells’ invasion ability. GraphPad Prism 7.0. was used for statistical analysis and graphical present cell number of migration and invasion. ∗means P <0.01 in t-test. CCAT1: Colon cancer-associated transcript 1. N.C. means negative control. N.S. means no significance; si-VIM mRNA: small interference RNA targeted VIM massage ribonucleic acid.
Fig 2: CCAT1 affected Vimentin protein level and enhanced cancer cells’ migration and invasion abilities. Recovery assay was performed to validate the binding between CCAT1 and Vimentin protein. CCAT1 up-expression plasmids and siRNA targeted to VIM mRNA were co-transfected into HeLa cells. (A) CCAT1 expression level increased obviously in CCAT1 plasmids transfected groups. CCAT1 over-expression did not affect the VIM mRNA level. VIM mRNA level decreased obviously in VIM-depleted groups without affecting CCAT1 expression level. (B) Western blotting examined the protein level of Vimentin. (C–F) Cell migration assays were performed for the above co-transfected HeLa cells. (D) Cell number was counted and statistically analyzed using GraphPad Prism software V7.0. VIM knocked down significantly weakened the migration ability of HeLa cells. This appearance could be reverted by increasing CCAT1 expression level by transfecting CCAT1 plasmids. (E) Cell invasion assays were performed for the above co-transfected HeLa cells. Cell numbers were counted and statistically analyzed using GraphPad Prism software V7.0. (F) VIM knocked down significantly weakened the invasion ability of HeLa cells. This appearance could be reverted by increasing CCAT1 expression level by transfecting CCAT1 plasmids. ∗means P < 0.05 and †means P < 0.01 in analysis of variance. CCAT1: Colon cancer-associated transcript 1. N.C. means negative control; mRNA: massage ribonucleic acid; GAPDH: Glyceraldehyde-3-Phosphate Dehydrogenase.
Supplier Page from Abcam for Anti-Vimentin antibody [RV203]