Anti-Vimentin antibody - Cytoskeleton Marker ab45939 from Abcam

Product Specs
ItemAnti-Vimentin antibody - Cytoskeleton Marker
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab45939
Antibody TypePrimary
Quantity100 UG
HostRabbit
ClonalityPolyclonal
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
IsotypeIgG
Conjugate/TagUnconjugated
Concentration0.9 mg/ml
Gene NameVIM
ValidationKnockOut Validated
ReactivityHuman, Mouse, Rat
TargetVimentin

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) SP1/RNASEH2A accelerates the development of hepatocellular carcinoma by regulating EMTHeliyonAugust 1, 2023Yunhe Hao, Rui Zou, Jiashou Tao, Manfei Jiang, Duo Liab235941, Abcam), N-cadherin (1:1000, ab245117, Abcam), Snail2 (1:1000, ab180714, Abcam), Vimentin (1:1000, ab45939, Abcam) and GAPDH (1:5000, ab8245, Abcam), and then with the corresponding secondary antibody. Enhanced

(2) Cytoplasmic HIF-2α as tissue biomarker to identify metastatic sympathetic paragangliomaScientific reportsJuly 18, 2023Karakaya S, Gunnesson L, Elias E, Martos-Salvo P, Robledo M, Nilsson O, Wängberg B, Abel F, Påhlman S, Muth A, et al.(SYP, M0776, Agilent, 1:200 + Linker), tyrosine hydroxylase (TH, ab75875, Abcam, 1:300), vimentin (ab45939, Abcam, 1:200), HIF-1α (610959, BD Biosciences, 1:50 + Linker), HIF-2α (A700-003, Bethyl Labs, 1:200

(3) Arctigenin hinders the invasion and metastasis of cervical cancer cells via the FAK/paxillin pathwayHeliyonJune 1, 2023Dan Liao, Yanyan Liu, Cuifen Li, Bin He, Guanghui Zhou, Yejia Cui, Haohai Huangantibodies against cleaved-caspase 3 (ab2302, 1:200, Abcam), E-cadherin (ab40772, 1:20000, Abcam), Vimentin (ab45939, 1:1000, Abcam), N-cadherin (ab18203, 1:1000, Abcam), FAK (ab40794, 1:2000, Abcam), phospho FAK (p-FAK,

(4) Temporal Dynamics of Metabolic Acquisition in Grafted Engineered Human Liver TissueAdvanced BiologyMay 1, 2023Chelsea L. Fortin, Tara N. McCray, Sarah H. Saxton, Fredrik Johansson, Christian B. Andino, Jonathan Mene, Yuliang Wang, Kelly R. Stevensspecies,Dilution CK18,Agilent,M701029–2,Mouse,1:100 CK19,Abcam,ab52625,Rabbit,1:250 Albumin,Bethyl,A80–129A,Goat,1:100 VIM,Abcam,ab45939,Rabbit,1:1000 CD31,Agilent,M0823,Mouse,1:20 ARG1,Sigma,HPA003595,Rabbit,1:400 CYP2A6,Sigma,HPA047262,Rabbit,1:200 ASS1,Sigma,HPA020934,Rabbit,1:200 HAL,Sigma,HPA038547,Rabbit,1:200 GS,Millipore,MAB302,Mouse,1:100 CYP2E1,Abcam,ab28146,Rabbit,1:100 Hu

(5) ADAMTS1 induces epithelial-mesenchymal transition pathway in non-small cell lung cancer by regulating TGF-βAging (Albany NY)March 31, 2023Xueqian Hu, Chunqi Jiang, Ning Hu, Shanyi Hongmembranes were blocked with 5% non-fat dry milk powder for 1h at 37° C. Anti-ADAMTS1 antibody (1:500; ab45939; Abcam, Cambridge, UK), anti-TGF-β antibody (1:500; 3711S, Cell Signaling Technology, Inc), anti-E-cadherin

(6) Celastrol Alleviates Corneal Stromal Fibrosis by Inhibiting TGF-β1/Smad2/3-YAP/TAZ Signaling After Descemet Stripping Endothelial KeratoplastyInvestigative Opthalmology & Visual ScienceMarch 3, 2023Ruixing Liu, Jingguo Li, Zhihua Guo, Dandan Chu, Chengcheng Li, Liuqi Shi, Junjie Zhang, Lei Zhu, Zhanrong Liexperiments. RCF Identification The F1 RCFs were incubated with primary antibodies against Vimentin (1:200, ab45939), Lumican (1:100, bs-5890R), and Keratocan (1:200, bs-11054R-PE) at 4°C overnight. Immunoreactivity

(7) Clinicopathologic Findings in Three Siblings With Geographic AtrophyInvestigative Opthalmology & Visual ScienceMarch 2, 2023Malia M. Edwards, D. Scott McLeod, Mengxi Shen, Rhonda Grebe, Janet S. Sunness, Imran A. Bhutto, Erin McDonnell, Alexandra M. Pado, Giovanni Gregori, Philip J. Rosenfeld, et al.Antibody,Source,Catalog #,Dilution Rb-a-GFAP,Dako,Z0334,1:200 Rb-a-vimentin,Abcam,AB45939,1:200 Ck-a-GFAP,Millipore/Sigma,AB5541,1:500 Ck-a-vimentin,Millipore/Sigma,AB5733,1:500 Rb-a-laminin,Millipore/Sigma,L-9393,1:200 Rb-a-CD44,Abcam,,1:200 UEA

(8) HIF-1α accumulation in response to transient hypoglycemia may worsen diabetic eye diseaseCell reportsJanuary 31, 2023Guo C, Deshpande M, Niu Y, Kachwala I, Flores-Bellver M, Megarity H, Nuse T, Babapoor-Farrokhran S, Ramada M, Sanchez J, et al.polyclonal anti-GFAP,Agilent DAKO,Cat# GA524; RRID:AB_2811722 Rabbit polyclonal anti-Vimentin,Abcam,Cat# ab45939; RRID:AB_2257290 Mouse monoclonal anti-PAX-6,DSHB,Cat# AB528427; RRID:AB_528427 Rabbit polyclonal

(9) Polyploid/Multinucleated Giant and Slow-Cycling Cancer Cell Enrichment in Response to X-ray Irradiation of Human Glioblastoma Multiforme Cells Differing in Radioresistance and TP53/PTEN StatusInternational Journal of Molecular SciencesJanuary 8, 2023Lina Alhaddad, Roman Chuprov-Netochin, Margarita Pustovalova, Andreyan N. Osipov, Sergey LeonovScientific™ Waltham, MA, USA), the membrane was incubated with primary anti-vimentin antibody (1 µg/mL, ab45939, Abcam, Cambridge, MA, USA), anti-E-cadherin antibody [EP700Y] (1:1000, ab40772, Abcam, Cambridge, MA,

(10) DOT1L regulates chamber-specific transcriptional networks during cardiogenesis and mediates postnatal cell cycle withdrawalNature communicationsDecember 2, 2022Cattaneo P, Hayes MGB, Baumgarten N, Hecker D, Peruzzo S, Aslan GS, Kunderfranco P, Larcher V, Zhang L, Contu R, et al.created by the NICHD of the NIH and maintained at The University of Iowa), anti-Vimentin (1:100 Abcam #ab45939), anti-TNNT (1:100 Thermo Fisher #MA5-12960), anti-PDGFR-α (1:200 R&D Systems #AF1062), anti-Collagen

(11) Multiparametric MRI identifies subtle adaptations for demarcation of disease transition in murine aortic valve stenosisBasic Research in CardiologyDecember 1, 2022Christine Quast et al.Roth), 0.1% saponin (Sigma) in PBS for 1 h at room temperature. Primary anti-vimentin antibody (Ab), ab45939 10 µg/ml; primary anti-α-smooth muscle actin (α-SMA) Ab, ab5694, 0.2 mg/ml; primary CD31 Ab, ab28364,

(12) The E3 ubiquitin ligase WWP2 regulates pro-fibrogenic monocyte infiltration and activity in heart fibrosisNature communicationsNovember 30, 2022Chen H, Chew G, Devapragash N, Loh JZ, Huang KY, Guo J, Liu S, Tan ELS, Chen S, Tee NGZ, et al.(#A302-936A, Bethyl Laboratories, 1:500), anti-ACTA2 (#A5228, Sigma-Aldrich, 1:10,000), anti-Vimentin (#ab45939, Abcam, 1:500), anti-Periostin (#NBP1-30042, Novus Bio, 1:500), anti-Fibronectin (#SAB4500974, Sigma-Aldrich,

(13) Mineralocorticoid receptor antagonists and glucocorticoids differentially affect skeletal muscle inflammation and pathology in muscular dystrophyJCI insightOctober 10, 2022Howard ZM, Gomatam CK, Rabolli CP, Lowe J, Piepho AB, Bansal SS, Accornero F, Rafael-Fortney JA.CD11b antibody (BD Pharmigen, 550282) and 1:600 rabbit anti–mouse polyclonal vimentin antibody (Abcam, ab45939); they were then incubated with 1:200 Alexa Fluor 488–conjugated chicken anti-rat (Invitrogen, A-21470)

(14) Ezh2 Inhibits Replicative Senescence of Atrial Fibroblasts Through Promotion of H3K27me3 in the Promoter Regions of CDKN2a and Timp4 GenesJournal of Inflammation ResearchAugust 16, 2022Yingze Li, Guojian Fang, Wei Cao, Jiali Yuan, Shuai Song, Hong Peng, Yuepeng Wang, Qunshan Wangcells were incubated with mouse antibody against H3K27me3 (ABclonal, A16199, 1:200), vimentin (Abcam, ab45939, 1:200), CDKN2A/p16INK4a (Abcam, ab211542, 1:200) and p21 (Abcam, ab188224, 1:200) at 4 °C overnight,

(15) Cancer-Associated Fibroblasts in a 3D Engineered Tissue Model Induce Tumor-like Matrix Stiffening and EMT TransitionCancers (Basel)August 5, 2022Martial Millet, Enola Bollmann, Cassandra Ringuette Goulet, Geneviève Bernard, Stéphane Chabaud, Marc-Étienne Huot, Frédéric Pouliot, Stéphane Bolduc, François Bordeleaupan-anti-Cytokeratin AE1/AE3 (#MAB3412) were from MilliporeSigma (Billerica, MA, USA); Rabbit anti-vimentin (#ab45939), Rabbit anti-MMP9 (#ab76003) were from Abcam (San Francisco, CA, USA). Secondary antibodies used were

(16) A novel nude mouse model for studying the pathogenesis of endometriosisExperimental and therapeutic medicineAugust 1, 2022Li LP, Li ZM, Wang ZZ, Cheng YF, He DM, Chen G, Cao BN, Zou Y, Luo Y.(1:250 dilution, 18008-1-AP, ProteinTech Group, Inc.) and rabbit anti-human vimentin (1:500 dilution, ab45939, Abcam) were used to identify stromal and epithelial cells and incubated overnight at 4˚C. The slides

(17) Chitosan Tubes Inoculated with Dental Pulp Stem Cells and Stem Cell Factor Enhance Facial Nerve-Vascularized Regeneration in RabbitsACS omegaJune 7, 2022Mu X, Liu H, Yang S, Li Y, Xiang L, Hu M, Wang X.with normal sheep serum, the samples were incubated with primary antibodies against vimentin (Abcam, ab45939, 1:200) and a second antibody (Invitrogen, a48282, 1:400). Next, they were visualized using a DAB chromogenic

(18) Immunomodulation of Acellular Dermal Matrix Through Interleukin 4 Enhances Vascular InfiltrationAnnals of Plastic SurgeryJune 1, 2022Patrick S. Cottler, Hannah Kang, Victoria Nash, Lisa Salopek, Anthony C. Bruce, Kara L. Spiller, Chris A. Campbellhematoxylin and eosin (H&E), and antibodies targeting vimentin, a marker of fibroblast lineage cells (ab45939; Abcam, Cambridge, United Kingdom) at a dilution of 1:200, α-smooth muscle actin (αSMA), a marker of

(19) Bladder cancer cell lines adapt their aggressiveness profile to oxygen tensionOncology LettersMay 20, 2022Stéphane Chabaud, Ève Pellerin, Christophe Caneparo, Cassandra Ringuette‑goulet, Frédéric Pouliot, Stéphane BolducMerck KGaA,"1/20,000","1/20,000",A5441 E-Cadherin,Mouse,Abcam,"1/1,000","1/1,000",Ab1416 EpCAM,Rabbit,Abcam,1/500,"1/1,000",Ab32392 N-Cadherin,Mouse,MilliporeSigma,"1/1,000","1/1,000",05-915 TSP-1,Rabbit,"Cell Signaling Technology, Inc.","1/1,000","1/1,000",37879 Vimentin,Rabbit,Abcam,"1/2,500","1/2,000",Ab45939 Antibodies used for western blotting. EpCAM, epithelial cell adhesion molecule; TSP-1, thrombospondin-1.

(20) Epithelial-mesenchymal transition and bi- and multi-nucleated trophoblast cell formation in ovine conceptuses during the peri-implantation periodThe Journal of reproduction and developmentApril 1, 2022Yamada A, Ohtsuki K, Shiga N, Green JA, Matsuno Y, Imakawa K.cytokeratin antibody (1:1000, Z0622; Dako, Glostrup, Denmark), rabbit anti-human VIM antibody (1:1000, ab45939; Abcam, Tokyo, Japan), rabbit anti-human E-cadherin (CDH1) antibody (1:1000, #3195; Cell Signaling Technology,

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12/22/2022Antibody for Staining Mesenchymal Cells Manuel Jr VidalWe are culturing mesenchymal cells in microfluidic devices. This antibody stains them well, with spindle shaped morphology clearly visible and brightly lit.

Anti-Vimentin antibody - Cytoskeleton Marker from Abcam

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Anti-Vimentin antibody - Cytoskeleton Marker from Abcam

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