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IFN-gamma Sandwich ELISA Kit
(1)
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R&D Systems, a Bio-Techne Brand
May 09, 2018
My goal was to compare the protein expression of IFN-y in the brains of wt and our newly generated mutant mouse strain. We selected this ...
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Detecting CD56
(1)
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BD Biosciences
May 09, 2018
We do a lot of NK cell detection. We wanted to test a few CD56 antibodies and find the "best" one which would give us the easiest resolution...
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Great 4°C Fridge for Lab Reagents
(1)
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Fisher Scientific
May 09, 2018
A great reliable fridge with dual clear glass doors that allow you to easily find and locate reagents. The fridge also carries several ...
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Flow Cytometry Marker For Long Term Hematopoietic Stem Cells
(1)
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BioLegend
May 08, 2018
We used this marker in the identification and isolation of murine long term hematopoietic stem cells from bone marrow. This antibody labels ...
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E.N.Z.A Total RNA Kit I
(1)
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Omega Bio-Tek, Inc.
May 08, 2018
My goal is to determine whether there is a differential expression of key genes in a mouse model of multiple sclerosis. I used this kit to ...
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Fast and Accurate qPCR Machine
(1)
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Agilent Technologies
May 08, 2018
Like any other molecular biology laboratory, the qRT-PCR machine is an integral part of our laboratory. We routinely use RT-PCR to confirm ...
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Restriction Cloning Using SpeI-HF
(1)
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New England Biolabs
May 08, 2018
I have used SpeI-HF restriction enzyme from NEB to digest the plasmid called pSSPgl of a size 24 Kb. Digestion resulted in 23 and 1 Kb DNA ...
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Transformation Of Expression Plasmid Constructs
(1)
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Thermo Fisher Scientific
May 07, 2018
I have constructed a expression plasmid called pACYC-cadA and transformed into to Invitrogen's One Shot Top10 chemically competent cells by ...
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Good Depletion Kit
(1)
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BioLegend
May 07, 2018
The general aim is to explore some rare cells in bone marrow that are CD45-. CD45 depletion was performed to remove most cells unwanted. The...
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Quick & Easy Viral Titer Estimate
(1)
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May 07, 2018
Recently used the Clontech Lenti-X go six to qualify my supernatants for viral titer. They are super easy to use and interpret and very were...
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Capture ELISA Kit To Measure Human IL-8 (CXCL8)
(1)
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May 07, 2018
The interleukin IL-8 (CXCL8) is one of the major cytokines involved in the regulation of inflammatory responses, including neutrophil ...
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Antibody For Venous Marker
(1)
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R&D Systems, a Bio-Techne Brand
May 04, 2018
This antibody was used to detect venous endothelial cells. We differentiated iPSCs to endothelial cells and cultured these endothelial cells...
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Great Ultrapure Water and Decently Priced
(1)
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ELGA LabWater
May 04, 2018
The flex 4 works great, spare parts are reasonably priced, and it looks very fancy! Great little machine that gives water with less than ...
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Diagnostic Restriction Mapping The Plasmid Construct With AatII
(1)
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New England Biolabs
May 04, 2018
I have constructed a plasmid called pTS30-flaB of a size 7.13 kb. The plasmid was prepped from the transformed E. coli cells and was ...
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Staining Human T Cells With Anti-Ki67 Alexa647
(1)
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BioLegend
May 04, 2018
This antibody was used along with 7-AAD for cell cycle analysis in stimulated human T cells. It was chosen because it is a commonly used ...
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Anti-CD137 Agonistic Antibody Treatment
(1)
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Bio X Cell
May 03, 2018
Agonistic anti-CD137 antibodies have been already reported to induce T cell mediated antitumor immunity. The aim of our experiments was to ...
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FasL Antibody
(1)
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Cell Signaling Technology
May 03, 2018
This antibody was used in a study that investigated the role of ALOX15 and its product 13-HODE in alcoholic fatty liver. It has been ...
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Staining Human T Cells With Anti-CD25 APC
(1)
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BioLegend
May 03, 2018
This antibody was used as a T cell activation marker in stimulated human T cells. It was chosen because it is a commonly used clone for CD25...
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Checking Adaptor Ligated Whole Genomic DNA Sequencing Library Fragment Size
(1)
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Agilent Technologies
May 03, 2018
I have sheared whole genomic DNA sequencing library to 400 bp and ligated them with Illumina sequencing adaptors. After ligation and clean ...
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Diagnostic Restriction Mapping Of The Plasmid Construct With XhoI
(1)
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New England Biolabs
May 02, 2018
I have designed and constructed a plasmid, pET20-cadA of size 6130 bp. After transforming this plasmid into E. coli BL21 cells, positive ...
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Clear Resolution Of Live Apoptotic And Non-Apoptotic Cells
(1)
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BioLegend
May 02, 2018
The aim was to study the role of various factors in B-cell survival in vitro. Annexin V staining was used to detect apoptotic cells.
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Excellent Rb1 Antibody
(1)
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Proteintech Group Inc
May 02, 2018
We have identified a class of ATPase inhibitors that show promise for treatment of ovarian cancer. We are currently evaluating what ...
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Cell Viability Dye
(1)
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Tonbo Biosciences
May 02, 2018
Cell viability dye is commonly used together with other fluorescent labeled antibody in flow cytometry analysis to discriminate dead cells ...
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Staining Human T Cells With Anti-CD69 APC
(1)
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BioLegend
May 02, 2018
This antibody was used as a T cell activation marker in stimulated human T cells. It was chosen because it is a commonly used clone for CD69...
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Staining Human T cells with anti-CD38 APC
(1)
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BioLegend
May 01, 2018
This antibody was used as a T cell activation marker in stimulated human T cells. It was chosen because it is a commonly used clone for CD38...
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Diagnostic Restriction Mapping Cloned Plasmid Constructs From E.Coli Cells Using NotI-HF
(1)
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New England Biolabs
May 01, 2018
I have constructed a plasmid called pET-evgS of size 7.12 kb. This plasmid was transformed into E. coli DH5 alpha cells and selected on agar...
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Anti-Human CD161 Antibody
(1)
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BioLegend
May 01, 2018
This antibody has been used in a flow cytometry-based study immunophenotyping PBMC populations obtained from cancer patients. The results ...
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Strong CD56 Signal
(1)
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BD Biosciences
May 01, 2018
We do a lot of NK cell staining. We needed a good CD56 antibody that gives us a good resolution of CD56(dim) from CD56(bright) and also from...
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Good Antibody To Detect Activated Src
(1)
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Cell Signaling Technology
May 01, 2018
The activity of the Src protein tyrosine kinase is important for regulation of cell growth, including proliferation of tumor cells. To ...
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Staining Human T Cells With Anti-HLA-DR APC
(1)
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BioLegend
April 30, 2018
This antibody was used to stain for HLA-DR expression on stimulated human T cells. It was chosen because it is a commonly used clone for HLA...
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Diagnostic Restriction Mapping Of The Plasmid Construct With NcoI-HF
(1)
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New England Biolabs
April 30, 2018
I have constructed a plasmid construct called pCU190_dgp of a size approximately 19 kb by Gibson assembly method. This plasmid prepped from ...
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Antibody Works Easily With Mouse CD8+ Cells
(1)
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BioLegend
April 30, 2018
One of the research focuses of our lab is to study the formation and maintenance of specific memory lymphocytes in different mouse organs. ...
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Isolating RNA From Exosomes
(1)
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Thermo Fisher Scientific
April 30, 2018
The Total Exosome RNA & Protein Isolation Kit can be used to isolate Total RNA and protein from exosomes. In our lab, we use the Total ...
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Capture ELISA Kit To Measure Human VEGF
(1)
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April 30, 2018
VEGF is one of the major growth factors that stimulate the formation of new (angiogenic) blood vessels. Measurement of VEGF production by ...
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CD32 PE flow antibody to stain FcgammaRII
(1)
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April 27, 2018
This antibody was used to determine FcgammaRII surface expression in human CD14 monocytes after blocking them with anti-CD32. The unblocked ...
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Excellent Kit To Measure Protein Concentration
(1)
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Thermo Fisher Scientific
April 27, 2018
Measurement of protein concentration is important in many biological applications, e.g. protein analysis by western blotting where equal ...
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Antibody For Hematopoietic Stem Cells
(1)
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BioLegend
April 27, 2018
This antibody can be used for the identification of hematopoietic stem cells isolated from the bone marrow. We use this antibody because of ...
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Good Western Blot Antibody
(1)
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Cell Signaling Technology
April 27, 2018
We have identified a class of ATPase inhibitors that show promise for treatment of ovarian cancer. We are currently evaluating what ...
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Diagnostic Restriction Mapping Of The Plasmid Construct With PvuII Enzyme
(1)
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New England Biolabs
April 27, 2018
I have constructed a plasmid called pUC19_Pgl2 of a size approximately 6.95 kb and transformed it into E.coli cells. After transformation, ...
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Staining Mouse Splenocytes with Anti-CD44 Pacific Blue
(1)
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BioLegend
April 27, 2018
This antibody was used to stain CD44+ cells in mouse splenocytes. It was chosen because it is a commonly used clone for CD44, PacBlue works ...
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CD144 (VE-cadherin) Antibody
(1)
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BioLegend
April 26, 2018
I was attempting to use this antibody for FACS analysis to validate an animal model. I performed a titration experiment using digested ...
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Excellent Antibody For Detection Of Full Length And Cleaved PARP-1 By Western Blotting
(1)
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Santa Cruz Biotechnology, Inc.
April 26, 2018
This antibody was used in a study where induction of apoptosis in human tumor cells was followed up by detection of cleaved PARP-1 in ...
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Good, Inexpensive Power Pack For Reproducible Westerns
(1)
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Bio-Rad
April 26, 2018
We have identified a class of ATPase inhibitors that show promise for treatment of ovarian cancer. We are currently evaluating what ...
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Isolating Cell Culture Exosomes
(1)
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Thermo Fisher Scientific
April 26, 2018
The ThermoFisher Exosome Isolation Reagent can be used to isolate small extracellular vesicles from cell conditioned media. The process is ...
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Staining Mouse Splenocytes With Anti-CD73 PerCP/Cy5.5
(1)
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BioLegend
April 26, 2018
This antibody was used to stain for CD73 expressing cells in mouse splenocytes. It was chosen because it is a commonly used clone for CD73, ...
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Amplification Of High Quality DNA Fragment With High Fidelity Using Pwo DNA Polymerase
(1)
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MilliporeSigma
April 26, 2018
I have used Pwo DNA polymerase for amplification of 6 kb DNA fragment with high fidelity for cloning into expression plasmid. PCR using this...
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Best Stress Granule Antibody
(1)
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Proteintech Group Inc
April 25, 2018
My study aims to understand how stress granules are linked to amyotrophic lateral sclerosis pathology. Specifically to understand the ...
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Diagnostic Restriction Mapping Of The Plasmid Construct With AflII Enzyme
(1)
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New England Biolabs
April 25, 2018
I have constructed a plasmid called pUC19_atpA of size 5113 bp. These constructed were transformed into E. coli DH5 alpha cells. Plasmid was...
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Excellent Antibody To Detect N-Cadherin By Western Blotting
(1)
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BD Biosciences
April 25, 2018
N-cadherin (neural cadherin) is commonly expressed by cancer cells of different tissue origin. This protein is important for homotypic (e.g....
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Use Of DRAQ5 To Label Nuclei In Human T Cells Prior To Amnis Straining Analysis
(1)
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BioLegend
April 25, 2018
DRAQ5 nuclear dye was used to label cell nuclei in order to use that as a marker of nuclei co-localization with other proteins.
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Cell Analysis Equipment
Cell Sorting Equipment
Cells and Strains
Centrifuge
Chemicals and Reagents
Chromatography Equipment
Columns
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Counter
Cytometer
Electrophoresis Equipment
Electroporation Equipment
ELISA Kits
Enzymes
Fluorescence Spectrophotometer
Freezer
Homogenizer
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Incubator
Injector
Kits
Labels / Dyes / Stains
Ladders and Markers
Light Sources
Liquid Handling Equipment
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Magnets
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Meter
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Primary Cells
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Purification Equipment
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Western Blot
Flow Cytometry
ELISA
Immunofluorescence
Immunoprecipitation
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Block/Neutralize/Inhibit
Cell Isolation
Dot Blot
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Purification
Staining
Cell Culture
DNA Purification
PCR
RNA Analysis
RNA Purification
qPCR
Clean-up
Microscopy
Cloning
RT-PCR
cDNA Synthesis
Transfection
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Cell Counting
Protein Purification
Next Gen Sequencing (NGS)
Library Construction
Genotyping
Gel Imaging
Protein Assay
Reporter Assay
Cell Lysis
Multiplex
Gel Staining
RNA-Seq
Live Cell Imaging
Cell Differentiation
Ligation
Concentration
HPLC
Sequencing
Chromatography
Mass Spectrometry
Labeling
Microarray
Mutagenesis
FISH
CRISPR
TUNEL Assay
In Vivo Imaging
EMSA
Cell Fractionation
Immunoassay
In Vitro Transcription
ChIP-Seq
Northern Blot
3D Imaging
RNAi
ELISPOT
PCR Cloning
Particle Analysis
Southern Blot
Protein Modification
DNA Analysis
Expression
Cell-Based Assay
Gene Editing
In Vivo Testing
Protein Labeling
Other
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Human
Mouse
Rat
Non-Human Primate
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Sheep
Hamster
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Horse
Monkey
C. elegans/Worm
Insect
Amoeba/Protozoa
Bacteria/Archaea
Arabidopsis
Yeast/Fungi
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Fish
Virus
Other Invertebrate
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Drosophila
Goat
Rhesus
Tissue
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Adipose
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Artery
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Blood Vessel
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Brain
Breast
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Cornea
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Dermis
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Endometrium
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Eye
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Hair
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Hippocampus
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In Vivo
Intestine
Kidney
Large Intestine
Liver
Lung
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Mammary Gland
Mouth
Muscle
Ovary
Pancreas
Peripheral Blood
Peritoneum
Placenta
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Rectum
Retina
Skeletal Muscle
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Spinal Cord
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Teeth
Testis
Thymus
Thyroid Gland
Tonsil
Trachea
Umbilical Cord
Umbilical Vein
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Vein
Cell Type
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Adipocyte
Astrocyte
B Cell
CD8+ T Cell
Competent Cells
Dendritic Cell
Embryonic Stem Cell
Endothelial Cell
Eosinophil
Epithelial Cell
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Helper T Cell
Hematopoietic Stem Cell
Hepatocyte
HUVEC
Induced Pluripotent Stem Cell
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Leukocytes
Lymphocyte
Macrophage
Mesenchymal Stem Cell
Microglia
Microvascular Endothelial Cell
Monocyte
Myoblasts
Myocyte
Neuron
Neutrophil
NK Cell
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Oligodendrocytes
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PBMC
Platelets
Progenitor Cell
Red Blood Cell
Regulatory T Cells
Skeletal Muscle Cell
Smooth Muscle Cell
Splenocyte
Stem Cell
Stromal Cell
Synoviocyte
T Cell
Thymocytes
Tumor Cells
Cell Line
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HeLa
PC3 (PC-3)
HEK293
HEK293T (HEK-293T)
CHO
Jurkat
3T3
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HepG2
THP-1
U2OS
PC12 (PC-12)
HL60 (HL-60)
A375 (A-375)
MOLT4 (MOLT-4)
MCF-7
A431
MDA-MB-231
A549
N2a
Daudi
HCT-116
Raji
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