Anti-Mouse CD8a Antibody

June 22, 2018

Medicine
Queen's University
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC/Cy7 anti-mouse CD8a Antibody

Catalog Number:

100713

We use this antibody to detect CD8+ T cells in mouse samples by flow cytometry. The antibody yields excellent staining, and will work in a variety of staining protocols including fix/perm. The results are always reliable.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse blood, spleen, lung, bone marrow

Primary Incubation

1:400 dlution, 30 min at 4C

Blocking Agent

Fc block (Anti CD16/CD32)

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometer

Results Summary

This antibody gives great results. We are able to reliably identify CD8+ T cells in a variety of protocols.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good reliable staining

The Bad

None

The Bottom Line

Excellent antibody.

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