Good Flow Cytometer

July 06, 2018

University of Pittsburgh
Neurosurgery

Overall

Performance

Ease-of-Optimization

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Company:

Beckman Coulter

Product Name:

CYTOFLEX FLOW CYTOMETER

Catalog Number:

B53037

We purchased this instrument few months back. It is giving us very good performance with nice sensitivity. The instrument is small and takes very minimal lab space with low maintenance. The price of the instrument is very much reasonable compared to other similar instrument. The advanced Photodiode (APD) detectors provide high sensitivity and unique compensation workflow that is easy to work. I will highly recommend this product for labs needing Flow cytometry for their regular use.

Experimental Design and Results Summary

Application

FACS or Flow cytometry

Protocol Overview

Followed mostly eBiosciences antibody protocol as described in the Thermoscientific web page, http://www.thermofisher.com/us/en/home/references/protocols/cell-and-tissue-analysis/protocols/staining-cell-surface-targets-flow-cytometry.html#suspensions

Tips

It is better to keep the flow rate at medium not at high.

Features Summary

The advanced Photodiode (APD) detectors provide high sensitivity and unique compensation workflow that is easy to work.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

The advanced Photodiode (APD) detectors provide high sensitivity and unique compensation workflow that is easy to work.

The Bad

The instrument is not good for big and heterogeneous cells.

The Bottom Line

We purchased this instrument few months back. It is giving us very good performance with nice sensitivity. The instrument is small and take very minimum lab space with low maintenance. The price of the instrument is very much reasonable compare to other similar instrument. The advanced Photodiode (APD) detectors provide high sensitivity and unique compensation workflow that is easy to work. However, the instrument is not good for big and heterogeneous cells. I will highly recommend this product for labs needing Flow cytometry for their regular use.

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