Cell Signaling Technology
Phospho-Rb (Ser780) (D59B7)
8180
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We are interested in mechanisms of resistance to targeted therapies in ovarian cancer. This experiment was designed to determine differential Rb1 protein expression and post-translational modifications in ovarian cancer cells lines resistant to a therapy, compared with therapy-sensitive controls.
Western Blot
Human ovarian cancer cell line lysates
1:1000 dilution in 5% milk in TBS-T, overnight at 4 degrees C
5% milk in TBS-T
1:10,000 in 5% milk in TBS-T, 30 minutes at RT
N/A
Chemiluminescence substrate for HRP-labeled secondary antibodies
We observed multiple bands on the blot, which may be due to non-specificity of the antibody or modified protein (identity of bands remains unclear). The staining was very strong, suggesting the antibody can be further diluted to help more clearly reflect differences (if any) in band intensity.
Very strong bands observed
Multiple nonspecific bands observed
Great antibody that can be used at dilute concentrations. However, it is unclear if the observed bands are biologically relevant or an artifact.