Anti Mouse CD45.1 Antibody

July 02, 2018

BlueskyReddit
Immunology
Gladstone Institutes
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

FITC Mouse Anti-Mouse CD45.1

Catalog Number:

553775

Our research is focused on enhancing CD8+ T cell functionality for immunotherapeutic purposes. We were interested in phenotyping CD8+ P14 transgenic T cells based on their congenic markers. This antibody is very reliable and provides robust data by flow cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse PBMCs

Primary Incubation

15-20 minutes @ 4 degrees centigrade

Blocking Agent

Fc Block (anti CD16/32)

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry on LSRII or Aria

Results Summary

Antibody robustly detects Congenically distinct lymphocytes. Shown below are congenitally marked CD8+ T cells that are stained for CD45.1 and CD45.2.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Reliable always works with short incubation times

The Bad

N/A

The Bottom Line

Reliable always works for quick analysis!

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