Cell Signaling Technology
Phospho-RB (Ser807/811) (D20B12) XP
8516
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We are interested in mechanisms of resistance to targeted therapies in ovarian cancer. This experiment was designed to determine differential Rb1 protein expression and post-translational modifications in ovarian cancer cells lines resistant to a therapy, compared with therapy-sensitive controls.
Western Blot
Human ovarian cancer cell line lysates
1:1000 dilution in 5% BSA in TBS-T, overnight at 4 degrees C
5% BSA in TBS-T
1:10,000 in 5% BSA in TBS-T, 30 minutes at RT
N/A
Chemiluminescence substrate for HRP-labeled secondary antibodies
Clean bands at 106 kDa, which is the correct predicted sized of Rb1 protein. Non-specific bands (one unidentified, one at 40 kDa that is likely actin) were observed, but don't interfere with interpretation of data.
Nice, clear bands at correct predicted size
Non-specific bands, but they don't interefere with interpretation of standard Western blot
Good antibody for Western, easy to optimize.