KIF15 Antibody

July 05, 2018

BlueskyReddit
Pathology
University of Kansas Medical Center
Postdoctoral Fellow

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Quality of Results

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Company:

Santa Cruz Biotechnology

Product Name:

KIF15 Antibody (36-I)

Catalog Number:

sc-100948

The purpose of this experiment was to determine expression of KIF15 in a disease-free ovarian epithelial cell line, as well as in the same cell line transfected with over-expression vectors.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human ovarian epithelium cell line lysates

Primary Incubation

1:200 dilution in 5% milk in TBS-T, overnight at 4 degrees C

Blocking Agent

5% milk in TBS-T

Secondary Incubation

1:10,000 in 5% milk in TBS-T, 30 minutes at RT

Tertiary Incubation

N/A

Detection

Chemiluminescence substrate for HRP-labeled secondary antibodies

Results Summary

We observed a number of non-specific bands including a strong band at 40 kDa which is likely beta-actin.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Free samples can be obtained to test the antibody in trial assays before purchase

The Bad

The non-specific bands were stronger than the likely specific band (KIF15 at 160 kDa)

The Bottom Line

Would not purchase this antibody due to non-specific staining.

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