TCR Beta Chain Flow Cytometry Antibody

Immunology
Gladstone Institutes
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

PE-CF594 anti-mouse TCR β chain [H57-597]

Catalog Number:

562841

Our research is focused on enhancing CD8+ T cell functionality for immunotherapeutic purposes. We were interested in phenotyping CD8+ T cell to evaluate their proliferation and used anti-TCR-beta in conjunction with anti-CD8 to identify our CD8+ T cells. This antibody is very reliable and provides robust data by flow cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse Splenocytes

Primary Incubation

30 minutes @ 4 degrees centigrade

Blocking Agent

Fc Block

Secondary Incubation

Not applicable

Tertiary Incubation

Not applicable

Detection

Flow Cytometry using LSRII

Results Summary

This antibody robustly defines T cells from mouse splenocytes/blood

DOI or PMID #

Not applicable

Additional Notes

Not applicable

Related Categories

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Summary

The Good

Always guaranteed to work!

The Bad

Not applicable

The Bottom Line

Perfect- reliable antibody

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