Anti-Mouse CD49b Antibody

Medicine
Queen's University
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE/Cy7 anti-mouse CD49b (pan-NK cells) Antibody

Catalog Number:

108921

This antibody is used extensively in flow cytometry panels to identify NK cells. The antibody gives a bright, robust signal. When gating on conventional NK cells (CD3-NKp46+) this antibody will usually bind more than 90% of cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse blood, spleen, lung, bone marrow

Primary Incubation

1:200 dilution, 30 min at 4C

Blocking Agent

Fc block (Anti CD16/CD32)

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometer

Results Summary

When gating on CD45+CD3-NKp46+ cells in the lymphocyte gate, this antibody will bind greater than 90% of all cells in mouse blood, spleen, and lung.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Bright, robust signal.

The Bad

None

The Bottom Line

Highly recommend this antibody for flow cytometry.

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